Human HK2 ELISA Kit | HK2 elisa kit

Human HK2 (Hexokinase 2) ELISA Kit

Cat. Num. AAA2515866

• Gene Names: ATP2A2; DD; DAR; ATP2B; SERCA2
• Reactivity: Human
• Synonyms: HK2; Human HK2 (Hexokinase 2) ELISA Kit; HK2 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinantHumanHK2. No significant cross-reactivity or interference between HumanHK2 and analogues was observed.
• Sequence Length: 997

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 125-8000pg/mL
• Sensitivity: Min: 75pg/mL; Max: 8000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HK2 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanHK2concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HK2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HK2and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HK2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HK2.You can calculate the concentration of HK2in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 306851
• NCBI GeneID: 488
• Molecular Weight: 109,734 Da
• NCBI Official Full Name: HK2
• NCBI Official Synonym Full Names: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• NCBI Official Symbol: ATP2A2
• NCBI Official Synonym Symbols: DD; DAR; ATP2B; SERCA2
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; SR Ca(2+)-ATPase 2; calcium pump 2; calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; cardiac Ca2+ ATPase; endoplasmic reticulum class 1/2 Ca(2+) ATPase
• UniProt Protein Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• Protein Family: Probable histidine kinase
• UniProt Gene Name: ATP2A2
• UniProt Synonym Gene Names: ATP2B; SERCA2; SR Ca(2+)-ATPase 2
• UniProt Entry Name: AT2A2_HUMAN

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

SERCA2: an intracellular pump located in the sarcoplasmic or endoplasmic reticula of muscle cells. Possesses a Ca(2+)-ATPase activity. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Two alternatively spliced isoforms have been described.

Protein type: Hydrolase; Transporter, ion channel; Cell adhesion; EC 3.6.3.8; Membrane protein, multi-pass; Transporter; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; sarcoplasmic reticulum; integral to plasma membrane; endoplasmic reticulum

Molecular Function: protein C-terminus binding; protein binding; enzyme binding; calcium-transporting ATPase activity; metal ion binding; calcium ion binding; ATP binding

Biological Process: cellular calcium ion homeostasis; ER-nuclear signaling pathway; endoplasmic reticulum calcium ion homeostasis; epidermis development; negative regulation of heart contraction; metabolic process; elevation of endoplasmic reticulum calcium ion concentration; positive regulation of heart rate; blood coagulation; cell adhesion; transmembrane transport; regulation of the force of heart contraction

Disease: Acrokeratosis Verruciformis; Darier-white Disease