Rabbit WDR77 Polyclonal Antibody | anti-WDR77 antibody

WDR77 Rabbit Polyclonal

Cat. Num. AAA7605736

• Gene Names: WDR77; p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: WDR77; Polyclonal Antibody; WDR77 Rabbit Polyclonal; anti-WDR77 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 330

• Applicable Applications for anti-WDR77 antibody: ELISA (EIA)
• Immunogen: WD repeat domain 77
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse heart using MBS7605736 (WDR77 antibody) at dilution of 1:200)

Western Blot (WB)

(Hela cells were subjected to SDS PAGE followed by western blot with MBS7605736 (WDR77 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 14495615
• NCBI GeneID: 79084
• Molecular Weight: 29,651 Da
• NCBI Official Full Name: WDR77 protein
• NCBI Official Synonym Full Names: WD repeat domain 77
• NCBI Official Symbol: WDR77
• NCBI Official Synonym Symbols: p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
• NCBI Protein Information: methylosome protein 50
• UniProt Protein Name: Methylosome protein 50
• Protein Family: Methylosome protein
• UniProt Gene Name: WDR77
• UniProt Synonym Gene Names: MEP50; WD45; MEP-50
• UniProt Entry Name: MEP50_HUMAN

NCBI Description

The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

WDR77: a WD40 repeat protein. Component of the methylosome, a 20S complex containing at least ICLN, SKB1 and WDR77. Interacts with SKB1 and with SM proteins. The methylosome may regulate an early step in the assembly of U snRNPs, possibly the transfer of Sm proteins to the SMN-complex. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: cytoplasm; cytosol; Golgi apparatus; nucleoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor transcription coactivator activity; methyl-CpG binding; protein binding; protein-arginine N-methyltransferase activity

Biological Process: peptidyl-arginine N-methylation; positive regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; spliceosomal snRNP biogenesis