Rabbit HK2 Polyclonal Antibody | anti-HK2 antibody

HK2 Antibody

Cat. Num. AAA5311190

• Gene Names: ATP2A2; DD; DAR; ATP2B; SERCA2
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Affinity Chromatography
• Synonyms: HK2; Polyclonal Antibody; HK2 Antibody; Rabbit Polyclonal HK2 Antibody; Polyclonal HK2 antibody; anti-HK2 antibody; HK-2; Muscle form hexokinase antibody; HK 2 antibody; HK II antibody; HK 2; HK-2 antibody; Hexokinase type II antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied in PBS buffer with 0.09% (W/V) Sodium Azide
• Concentration: 250 ug/ml (varies by lot)
• Sequence Length: 997

• Applicable Applications for anti-HK2 antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: ELISA: 0.25 ug/ml; IHC: 2.5-5.0 ug/ml; WB: 0.5-2.5 ug/ml
• Immunogen: HK2 antibody was raised in Rabbit using synthetic peptide selected from the N-terminal region of human HK2 as the immunogen.
• Cross-Reactivity: Human, Mouse
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Western Blot (WB)

(Western blot analysis of anti- HK2 to detect HK2 in A375 cell lysate.)

Related Product Information for anti-HK2 antibody

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.

Product Categories/Family for anti-HK2 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 306851
• NCBI GeneID: 488
• NCBI Official Full Name: HK2
• NCBI Official Synonym Full Names: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
• NCBI Official Symbol: ATP2A2
• NCBI Official Synonym Symbols: DD; DAR; ATP2B; SERCA2
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• Protein Family: Probable histidine kinase

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]