Western Blot (WB)
(Western blot analysis of Histone H2B in human PBMC cell lysate with IMG-359.)
Rabbit anti-Human Histone H2B Polyclonal Antibody | anti-H2B antibody
Histone H2B (CT)
Reactivity
Human
Applications
Western Blot
Purity
PurifiedPurified
Synonyms
Histone H2B; Polyclonal Antibody; Histone H2B (CT); Anti -Histone H2B (CT); anti-H2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Histone H2B (CT)
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Applicable Applications for anti-H2B antibody
Western Blot (WB)
Application Notes
Suitable for the detection of Histone H2B by Western blot. Human peripheral blood cell lysate or Jurkat cell lysate can be used as positive control. An approximately 15kD band is detected. Western blot analysis of Histone H2B in human PBMC cell lysate with antibody.
Dilution: Western blot: 2ug/ml
Dilution: Western blot: 2ug/ml
Immunogen
Synthetic peptide containing amino acid residues 111-125 of human Histone H2B.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of Histone H2B in human PBMC cell lysate with IMG-359.)
Western Blot (WB)
(Western blot analysis of Histone H2B in human PBMC cell lysate with IMG-359.)
Related Product Information for anti-H2B antibody
Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells.
Product Categories/Family for anti-H2B antibody
NCBI and Uniprot Product Information
Uniprot Description
Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
By similarity. SAAS SAAS000558
Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA
By similarity. RuleBase RU000451
Subcellular location: Nucleus
By similarity RuleBase RU000451.
Sequence similarities: Belongs to the histone H2B family. RuleBase RU000451
Similar Products
Product Notes
The Histone H2B h2b (Catalog #AAA622268) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B (CT) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for the detection of Histone H2B by Western blot. Human peripheral blood cell lysate or Jurkat cell lysate can be used as positive control. An approximately 15kD band is detected. Western blot analysis of Histone H2B in human PBMC cell lysate with antibody. Dilution: Western blot: 2ug/ml. Researchers should empirically determine the suitability of the Histone H2B h2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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