SDS-PAGE
C Reactive Protein (CRP) Recombinant Protein | CRP recombinant protein
Recombinant C Reactive Protein (CRP)
Gene Names
Crp; AI255847
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
C Reactive Protein (CRP); Recombinant C Reactive Protein (CRP); CRP recombinant protein
Host
E. coli
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01%skl, 5% Trehalose.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
HEDMFKKAFV FPKESDTSYV SLEAESKKPL NTFTVCLHFY TALSTVRSFS VFSYATKKNS NDILIFWNKD KQYTFGVGGA EVRFMVSEIP EAPTHICASW ESATGIVEFW IDGKAKVRKS LHKGYTVGPD ASIILGQEQD SYGGDFDAKQ SLVGDIGDVN MWDFVLSPEQ INTVYVGGTL SPNVLNWRAL NYKAQGDVFI KPQLWS
Applicable Applications for CRP recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Organism Species
Mus musculus (Mouse)
Source
Prokaryotic expression
Residues
His20~Ser225Tags
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
5.6
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
Gene Sequencing (Extract)
Gene Sequencing (Extract)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 29.8kDa
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
C-reactive protein
NCBI Official Synonym Full Names
C-reactive protein, pentraxin-related
NCBI Official Symbol
Crp
NCBI Official Synonym Symbols
AI255847
NCBI Protein Information
C-reactive protein; C-reactive protein, petaxin related
UniProt Protein Name
C-reactive protein
Protein Family
UniProt Gene Name
Crp
UniProt Synonym Gene Names
Ptx1
UniProt Entry Name
CRP_MOUSE
Uniprot Description
CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; growth cone; extracellular region; filopodium
Molecular Function: protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; metal ion binding; low-density lipoprotein binding; cholesterol binding; calcium ion binding
Biological Process: protein polymerization; positive regulation of nitric oxide biosynthetic process; negative regulation of vasodilation; wound healing; positive regulation of superoxide release; acute-phase response; negative regulation of superoxide release; complement activation, classical pathway
Research Articles on CRP
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Product Notes
The CRP crp (Catalog #AAA2009439) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's C Reactive Protein (CRP) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the CRP crp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HEDMFKKAFV FPKESDTSYV SLEAESKKPL NTFTVCLHFY TALSTVRSFS VFSYATKKNS NDILIFWNKD KQYTFGVGGA EVRFMVSEIP EAPTHICASW ESATGIVEFW IDGKAKVRKS LHKGYTVGPD ASIILGQEQD SYGGDFDAKQ SLVGDIGDVN MWDFVLSPEQ INTVYVGGTL SPNVLNWRAL NYKAQGDVFI KPQLWS. It is sometimes possible for the material contained within the vial of "C Reactive Protein (CRP), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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