Rabbit anti-Human Histone H2B Polyclonal Antibody | anti-HIST1H2BB antibody

Histone H2B, NT (HIST1H2BB, H2BFF, HIST1H2BE, Histone H2B type 1-B, Histone H2B.1, Histone H2B.f) (AP)

Cat. Num. AAA6306945

• Gene Names: HIST1H2BB; H2B.1; H2B/f; H2BFF
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Histone H2B; Polyclonal Antibody; NT (HIST1H2BB; H2BFF; HIST1H2BE; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f) (AP); HIST1H2BB; Histone H2B.f; anti-HIST1H2BB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HIST1H2BB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HIST1H2BB/HIST1H2BE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2BB/HIST1H2BE.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HIST1H2BB/HIST1H2BE Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HIST1H2BB/HIST1H2BE antibody detected the HIST1H2BB/HIST1H2BE protein (arrow).)

Western Blot (WB)

(Western blot analysis of HIST1H2BB/HIST1H2BE (arrow) using rabbit polyclonal HIST1H2BB/HIST1H2BE Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HIST1H2BB/HIST1H2BE gene.)

Related Product Information for anti-HIST1H2BB antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Product Categories/Family for anti-HIST1H2BB antibody

Antibodies; Histones

References

Kim, S.C., et al. Mol. Cell 23(4):607-618(2006). Pavri, R., et al. Cell 125(4):703-717(2006). Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006). Zhu, B., et al. Mol. Cell 20(4):601-611(2005). Golebiowski, F., et al. Mol. Cell. Biochem. 279 (1-2), 133-139 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 10800140
• NCBI GeneID: 3018
• NCBI Accession #: NP_066406.1
• NCBI GenBank Nucleotide #: NM_021062.2
• UniProt Accession #: P33778; Q4KN36
• Molecular Weight: 13,950 Da
• NCBI Official Full Name: histone H2B type 1-B
• NCBI Official Synonym Full Names: histone cluster 1, H2bb
• NCBI Official Symbol: HIST1H2BB
• NCBI Official Synonym Symbols: H2B.1; H2B/f; H2BFF
• NCBI Protein Information: histone H2B type 1-B; H2B histone family, member F; histone 1, H2bb; histone H2B.1; histone H2B.f
• UniProt Protein Name: Histone H2B type 1-B
• Protein Family: Histone
• UniProt Gene Name: HIST1H2BB
• UniProt Synonym Gene Names: H2BFF; H2B/f
• UniProt Entry Name: H2B1B_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.