Western Blot (WB)
(Sample Data: Western blot analysis of extract from 293 cells untreated or treated with UV, using Histone H2A.X antibody (Lane 1+2) and Anti-phospho-Histone H2A.X (Ser139) (Lane 3+4))
Rabbit Histone H2A.X Polyclonal Antibody | anti-phospho-HistoneH2A.X antibody
Anti-phospho-Histone H2A.X (Ser139)
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity chromatography
Synonyms
Histone H2A.X; Polyclonal Antibody; Anti-phospho-Histone H2A.X (Ser139); anti-phospho-HistoneH2A.X antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the Histone H2A.X protein phosphorylated at serine 139
Purity/Purification
Affinity chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-phospho-HistoneH2A.X antibody
Western Blot (WB)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of extract from 293 cells untreated or treated with UV, using Histone H2A.X antibody (Lane 1+2) and Anti-phospho-Histone H2A.X (Ser139) (Lane 3+4))
Western Blot (WB)
(Sample Data: Western blot analysis of extract from 293 cells untreated or treated with UV, using Histone H2A.X antibody (Lane 1+2) and Anti-phospho-Histone H2A.X (Ser139) (Lane 3+4))
Immunofluorescence (IF)
(Sample Data: Immunofluorescence staining of methanol-fixing HeLa cells using Anti-phospho-Histone H2A.X (Ser139))
Immunofluorescence (IF)
(Sample Data: Immunofluorescence staining of methanol-fixing HeLa cells using Anti-phospho-Histone H2A.X (Ser139))
Western Blot (WB)
(Sample Data: Western blot analysis of extracts from HT29 cells untreated (Lane 1) or treated with UV (Lane 2) using Anti-phospho-Histone H2A.X (Ser139))
Western Blot (WB)
(Sample Data: Western blot analysis of extracts from HT29 cells untreated (Lane 1) or treated with UV (Lane 2) using Anti-phospho-Histone H2A.X (Ser139))
Product Categories/Family for anti-phospho-HistoneH2A.X antibody
References
1. Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
2. Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6.
2. Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6.
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Product Notes
The phospho-HistoneH2A.X (Catalog #AAA515988) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-Histone H2A.X (Ser139) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A.X can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the phospho-HistoneH2A.X for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A.X, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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