anti-Human, Mouse HistoneH2A.X Antibody | anti-H2a/x antibody

HistoneH2A.X Phospho-Ser139) Antibody

Cat. Num. AAA8510157

• Gene Names: H2AFX; H2AX; H2A.X; H2A/X
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: HistoneH2A.X; HistoneH2A.X Phospho-Ser139) Antibody; anti-H2a/x antibody

• Reactivity: Human, Mouse
• Specificity: Histone H2A.X (Phospho-Ser139) antibody detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 143

• Applicable Applications for anti-H2a/x antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
• All Sites: Human: Ser139; Mouse: Ser139
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, untreated or treated with UV, using Histone H2A.X (Ab-139) antibody (Line 1 and 2) and Histone H2A.X (Phospho-Ser139) antibody (Line 3 and 4).)

References

Alpna Tyagi, et al. (2005) Carcinogenesis; 26: 1978-1987. James P, et al. (2002) Nucleic Acids Res; 30: 3698-3705. Mariana Yaneva, et al. (2005) Nucleic Acids Res; 33: 5320-5330. Jérôme Govin, et al. (2004) Eur. J. Biochem; 271: 3459-3469.

NCBI and Uniprot Product Information

• NCBI GI #: 4504253
• NCBI GeneID: 3014
• NCBI Accession #: NP_002096.1
• NCBI GenBank Nucleotide #: NM_002105.2
• UniProt Accession #: P16104; Q4ZGJ7; Q6IAS5
• Molecular Weight: 15,145 Da
• NCBI Official Full Name: histone H2AX
• NCBI Official Synonym Full Names: H2A histone family member X
• NCBI Official Symbol: H2AFX
• NCBI Official Synonym Symbols: H2AX; H2A.X; H2A/X
• NCBI Protein Information: histone H2AX
• UniProt Protein Name: Histone H2AX
• UniProt Gene Name: H2AFX
• UniProt Synonym Gene Names: H2AX; H2a/x

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]

Uniprot Description

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.