Rabbit Histone H2AK119ub Polyclonal Antibody | anti-H2AK119ub antibody

Histone H2AK119ub antibody (pAb)

Cat. Num. AAA389062

• Gene Names: HIST2H2AC; H2A; H2A/q; H2AFQ; H2A-GL101
• Reactivity: Human, Wide Range Predicted
• Applications: Dot Blot, Western Blot
• Purity: Affinity Purified
• Synonyms: Histone H2AK119ub; Polyclonal Antibody; Histone H2AK119ub antibody (pAb); anti-H2AK119ub antibody

• Host: Rabbit
• Reactivity: Human, Wide Range Predicted
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol, and 0.035% sodium azide. Sodium azide is highly toxic.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-H2AK119ub antibody: Dot Blot (DB), Western Blot (WB)
• Application Notes: Applications Validated:; DB: 1:1000; WB: 1:1000-1:2000 dilution; Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. For optimal results, we recommend the addition of 0.05% Tween-20 to all blocking solutions to reduce background.
• Immunogen: This antibody was raised against a branched peptide corresponding to the ubiquitin conjugation site at Lys119 of human Histone H2A.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

SDS-Page

(Histone H2AK119ub antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with antibody at a 1:500 dilution.)

Dot Blot (DB)

(Histone H2AK119ub antibody tested by Dot blot. Lane 1: unmodified Histone H2A. Lane 2: ubiquityl-lysine 119 H2A. Peptides were spotted on membrane and probed with antibody at a dilution of 1:1000.)

Related Product Information for anti-H2AK119ub antibody

Short Description: Our Histone H2AK119ub antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Background: In humans, attachment of ubiquitin to Histone protein 2A (H2A) on lysine residue 119 (H2AK119Ub) is one of the most prevalent histone marks, and is estimated to occur on 5-15% of histones genome-wide4. In general, H2AK119Ub is characteristic of repressed developmental genes, and is also tightly linked to levels of other histone marks such as lysine methylation and acetylation. H2AK119Ub is attached to histones by the Really Interesting New Gene (RING) ubiquitin E3-ligase Polycomb Repressive Complex 1 (PRC1), and removed by the Polycomb Repressive-Deubiquitinase (PR-DUB) complex, which plays a vital role in regulating H2AK119Ub histone marks, and mutations in human PR-DUB genes lead to a spectrum of malignancies.

Product Categories/Family for anti-H2AK119ub antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 24638446
• NCBI GeneID: 8338
• NCBI Accession #: NP_003508
• NCBI GenBank Nucleotide #: NM_003517
• UniProt Accession #: Q16777
• Molecular Weight: 14kDa
• NCBI Official Full Name: histone H2A type 2-C
• NCBI Official Synonym Full Names: histone cluster 2 H2A family member c
• NCBI Official Symbol: HIST2H2AC
• NCBI Official Synonym Symbols: H2A; H2A/q; H2AFQ; H2A-GL101
• NCBI Protein Information: histone H2A type 2-C
• UniProt Protein Name: Histone H2A type 2-C
• UniProt Gene Name: HIST2H2AC
• UniProt Synonym Gene Names: H2AFQ
• UniProt Entry Name: H2A2C_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. [provided by RefSeq, Aug 2015]

Uniprot Description

Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Subcellular location: Nucleus. Chromosome.

Post-translational modification: The chromatin-associated form is phosphorylated on Thr-121 during mitosis

Probable. Ref.6 Ref.7Deiminated on Arg-4 in granulocytes upon calcium entry.Monoubiquitination of Lys-120 (H2AK119Ub) by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3 (H3K27me). H2AK119Ub by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Ubiquitination at Lys-14 and Lys-16 (H2AK13Ub and H2AK15Ub, respectively) in response to DNA damage is initiated by RNF168 that mediates monoubiquitination at these 2 sites, and 'Lys-63'-linked ubiquitin are then conjugated to monoubiquitin; RNF8 is able to extend 'Lys-63'-linked ubiquitin chains in vitro. H2AK119Ub and ionizing radiation-induced 'Lys-63'-linked ubiquitination (H2AK13Ub and H2AK15Ub) are distinct events. Ref.8 Ref.10 Ref.11 Ref.14 Ref.15 Ref.17 Ref.18 Ref.20 Ref.21Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage

By similarity.Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Ref.19

Sequence similarities: Belongs to the histone H2A family.

Mass spectrometry: Molecular mass is 13890.8 Da from positions 2 - 129. Determined by ESI. Monoisotopic with N-acetylserine. Ref.12