Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse HDAC4 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Mouse Histone Deacetylase 4 Polyclonal Antibody | anti-HDAC4 antibody
Polyclonal Antibody to Histone Deacetylase 4 (HDAC4)
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse HDAC4 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse HDAC4 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue))
NCBI and Uniprot Product Information
Uniprot Description
HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
Protein type: Deacetylase; EC 3.5.1.98; Hydrolase; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 1|1 D
Cellular Component: A band; actomyosin; cytoplasm; cytosol; histone deacetylase complex; neuromuscular junction; nucleoplasm; nucleus; protein complex; sarcomere; transcriptional repressor complex; Z disc
Molecular Function: activating transcription factor binding; chromatin binding; DNA binding; histone deacetylase activity; histone deacetylase binding; hydrolase activity; identical protein binding; metal ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); potassium ion binding; protein binding; protein deacetylase activity; protein kinase binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor binding; transcription regulatory region DNA binding; zinc ion binding
Biological Process: chromatin organization; chromatin remodeling; covalent chromatin modification; histone deacetylation; negative regulation of cell proliferation; negative regulation of glycolytic process; negative regulation of myotube differentiation; negative regulation of osteoblast differentiation; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; osteoblast development; positive regulation of cell proliferation; positive regulation of lamellipodium assembly; positive regulation of neuron apoptotic process; positive regulation of protein sumoylation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; protein deacetylation; regulation of cardiac muscle contraction by calcium ion signaling; regulation of gene expression, epigenetic; regulation of protein binding; regulation of skeletal muscle fiber development; regulation of striated muscle cell differentiation; regulation of transcription, DNA-templated; response to denervation involved in regulation of muscle adaptation; response to drug; skeletal system development; transcription, DNA-dependent
Research Articles on HDAC4
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Product Notes
The HDAC4 hdac4 (Catalog #AAA2028345) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Histone Deacetylase 4 (HDAC4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone Deacetylase 4 can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2µg/mL Immunocytochemistry in formalin fixed cells: 5-20µg/mL Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL Immunohistochemistry in paraffin section: 5-20µg/mL Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the HDAC4 hdac4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone Deacetylase 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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