Western Blot (WB)
(Western Blot: Sample: Recombinant ITGaD, Mouse.)
Rabbit anti-Mouse Integrin Alpha D (ITGaD) Polyclonal Antibody | anti-ITGaD antibody
Polyclonal Antibody to Integrin Alpha D (ITGaD)
Gene Names
Itgad; Cd11d
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific Affinity Chromatography
Synonyms
Integrin Alpha D (ITGaD); Polyclonal Antibody; Polyclonal Antibody to Integrin Alpha D (ITGaD); anti-ITGaD antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a Rabbit polyclonal antibody raised against ITGaD. It has been selected for its ability to recognize ITGaD in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific Affinity Chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-ITGaD antibody
WB; IHC; ICC; IP
Application Notes
Western blotting: 0.5-2µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant ITGaD (Phe886~Glu1106) expressed in E.coli
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040354
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant ITGaD, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ITGaD, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: ITGaD knockout 293T cell lysate; Predicted MW: 128kDa Observed MW: 150kDa Primary Ab: 1ug/ml Rabbit Anti-Mouse ITGaD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: ITGaD knockout 293T cell lysate; Predicted MW: 128kDa Observed MW: 150kDa Primary Ab: 1ug/ml Rabbit Anti-Mouse ITGaD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Product Categories/Family for anti-ITGaD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
127,829 Da
NCBI Official Full Name
Integrin alpha-D
NCBI Official Synonym Full Names
integrin, alpha D
NCBI Official Symbol
Itgad
NCBI Official Synonym Symbols
Cd11d
NCBI Protein Information
integrin alpha-D
UniProt Protein Name
Integrin alpha-D
Protein Family
UniProt Gene Name
Itgad
Uniprot Description
ITGAD: Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. May play a role in the atherosclerotic process such as clearing lipoproteins from plaques and in phagocytosis of blood- borne pathogens, particulate matter, and senescent erythrocytes from the blood. Belongs to the integrin alpha chain family.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7|7 F3
Cellular Component: integral component of membrane; integrin complex; membrane
Molecular Function: metal ion binding; protein heterodimerization activity
Biological Process: activated T cell proliferation; cell adhesion; heterotypic cell-cell adhesion; integrin-mediated signaling pathway
Research Articles on ITGaD
Similar Products
Product Notes
The ITGaD itgad (Catalog #AAA2015653) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Integrin Alpha D (ITGaD) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin Alpha D (ITGaD) can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2µg/mL Immunocytochemistry in formalin fixed cells: 5-20µg/mL Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL Immunohistochemistry in paraffin section: 5-20µg/mL Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the ITGaD itgad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin Alpha D (ITGaD), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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