Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Gallus Tumor Necrosis Factor Alpha Polyclonal Antibody | anti-TNFa antibody
Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
Gene Names
LITAF; TNF-alpha
Reactivity
Gallus
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific Affinity Chromatography
Synonyms
Tumor Necrosis Factor Alpha; Polyclonal Antibody; Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa); anti-TNFa antibody
Host
Rabbit
Reactivity
Gallus
Clonality
Polyclonal
Specificity
The antibody is a Rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific Affinity Chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.65mg/ml (varies by lot)
Applicable Applications for anti-TNFa antibody
WB; IHC; ICC; IP
Application Notes
Western blotting: 0.5-2µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant TNFa (Arg1~Phe73) expressed in E.coli
Cross Reactivity
Chicken
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2038459
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
16,013 Da
NCBI Official Full Name
tumor necrosis factor-alpha, partial
NCBI Official Synonym Full Names
lipopolysaccharide induced TNF factor
NCBI Official Symbol
LITAF
NCBI Official Synonym Symbols
TNF-alpha
NCBI Protein Information
lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
UniProt Protein Name
Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
Protein Family
UniProt Gene Name
LITAF
UniProt Synonym Gene Names
SIMPLE; LPS-induced TNF-alpha factor homolog
Uniprot Description
Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role in the nucleus in regulating the expression of numerous cytokines.
Research Articles on TNFa
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Product Notes
The TNFa litaf (Catalog #AAA2027013) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) reacts with Gallus and may cross-react with other species as described in the data sheet. AAA Biotech's Tumor Necrosis Factor Alpha can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2µg/mL Immunocytochemistry in formalin fixed cells: 5-20µg/mL Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL Immunohistochemistry in paraffin section: 5-20µg/mL Optimal working dilutions must be determined by end user . Researchers should empirically determine the suitability of the TNFa litaf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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