Rabbit HGS Polyclonal Antibody | anti-HGS antibody

Phospho-HGS (Tyr216) Antibody

Cat. Num. AAA9612929

• Gene Names: HGS; HRS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: HGS; Polyclonal Antibody; Phospho-HGS (Tyr216) Antibody; Hepatocyte growth factor regulated tyrosine kinase substrate; Hepatocyte growth factor-regulated tyrosine kinase substrate; HGF-regulated tyrosine kinase substrate; HGNC:4897; HGS_HUMAN; Hrs; Human growth factor regulated tyrosine kinase substrate; Protein pp110; ZFYVE8; anti-HGS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-HGS (Tyr216) Antibody detects endogenous levels of HGS only when phosphorylated at Tyr216.; Tissue Specificity: Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-HGS antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human HGS around the phosphorylation site of Tyr216.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.Ubiquitinated. Ubiquitinated by ITCH.
• Subunit Structure: Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 (or probably STAM) and EPS15. Interacts with STAM. Interacts with STAM2 (By similarity). Interacts with EPS15; the interaction is direct, calcium-dependent and inhibited by SNAP25 (By similarity). Identified in a complex with STAM and LITAF. Found in a complex with STAM and E3 ligase ITCH and DTX3L. Interacts with E3 ligase DTX3L; the interaction brings together STAM and HSG, promotes their recruitment to early endosomes and decreases STAM and HGS ubiquitination by ITCH. Interacts with NF2; the interaction is direct. Interacts with ubiquitin; the interaction is direct (By similarity). Interacts with VPS37C. Interacts with SMAD1, SMAD2 and SMAD3 (By similarity). Interacts with TSG101; the interaction mediates the association with the ESCRT-I complex. Interacts with SNAP25; the interaction is direct and decreases with addition of increasing concentrations of free calcium (By similarity). Interacts with SNX1; the interaction is direct (By similarity). Component of a 550kDa membrane complex at least composed of HGS and SNX1 but excluding EGFR (By similarity). Interacts with TRAK1. Interacts with TRAK2 (By similarity). Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts (via UIM domain) with UBQLN1 (via ubiquitin-like domain) (By similarity). Interacts with ARRDC3. Identified in a complex containing at least ARRDC4, AVPR2 and HGS. Interacts with LAPTM4B; promotes HGS ubiquitination.
• Similarity: Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns (3)P-enriched membranes is substantially increased in acidic conditions.
• Subcellular Location: Cytoplasm. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side. Endosome>Multivesicular body membrane>Peripheral membrane protein.; Note: Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse brain, using Phospho-HGS (Tyr216) Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-HGS antibody

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

NCBI and Uniprot Product Information

• NCBI GI #: 4758528
• NCBI GeneID: 9146
• NCBI Accession #: NP_004703.1
• NCBI GenBank Nucleotide #: NM_004712.4
• UniProt Accession #: O14964; Q9NR36
• Molecular Weight: 86,192 Da
• NCBI Official Full Name: hepatocyte growth factor-regulated tyrosine kinase substrate
• NCBI Official Synonym Full Names: hepatocyte growth factor-regulated tyrosine kinase substrate
• NCBI Official Symbol: HGS
• NCBI Official Synonym Symbols: HRS
• NCBI Protein Information: hepatocyte growth factor-regulated tyrosine kinase substrate; protein pp110; human growth factor-regulated tyrosine kinase substrate
• UniProt Protein Name: Hepatocyte growth factor-regulated tyrosine kinase substrate
• Protein Family: Hepatocyte growth factor-regulated tyrosine kinase substrate
• UniProt Gene Name: HGS
• UniProt Synonym Gene Names: HRS
• UniProt Entry Name: HGS_HUMAN

NCBI Description

The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010]

Uniprot Description

Hrs: a zinc finger protein which is rapidly tyrosine phosphorylated in cells stimulated with various growth factors. Hrs mRNA is expressed in all the human adult and fetal tissues tested.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: intracellular membrane-bound organelle; early endosome membrane; early endosome; cytoplasm; cytosol; endosome; secretory granule

Molecular Function: protein domain specific binding; protein binding; metal ion binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; negative regulation of cell proliferation; protein targeting to lysosome; membrane invagination; endosome to lysosome transport; regulation of protein catabolic process; endosome transport; signal transduction; negative regulation of JAK-STAT cascade