Rabbit anti-Human HGS Polyclonal Antibody | anti-Hgs antibody

HGS, ID (HRS, Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate, HGNC:4897, Human Growth Factor Regulated Tyrosine Kinase Substrate, Protein pp110, ZFYVE8)

Cat. Num. AAA645057

• Gene Names: Hgs; Hgr; Hrs; ZFYVE8
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: HGS; Polyclonal Antibody; ID (HRS; Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate; HGNC:4897; Human Growth Factor Regulated Tyrosine Kinase Substrate; Protein pp110; ZFYVE8); Anti -HGS; anti-Hgs antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HGS. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Hgs antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.; Dilution: ELISA: 1:40,000; Western Blot: 1:500-1:1000; Immunofluorescence: 1:100-1:500; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human HGS.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Pancreas, Islets of Langerhans: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts from 293/HuvEc cells, using HGS Antibody. The lane on the right is treated with the synthesized peptide.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HuvEc cells, using HGS Antibody. The picture on the right is treated with the synthesized peptide.)

Related Product Information for anti-Hgs antibody

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

Product Categories/Family for anti-Hgs antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 226874952
• NCBI GeneID: 15239
• NCBI Accession #: NP_032270.3
• NCBI GenBank Nucleotide #: NM_008244.3
• UniProt Accession #: Q99LI8; Q61691; Q8BQW3
• Molecular Weight: 86,015 Da
• NCBI Official Full Name: hepatocyte growth factor-regulated tyrosine kinase substrate isoform 2
• NCBI Official Synonym Full Names: HGF-regulated tyrosine kinase substrate
• NCBI Official Symbol: Hgs
• NCBI Official Synonym Symbols: Hgr; Hrs; ZFYVE8
• NCBI Protein Information: hepatocyte growth factor-regulated tyrosine kinase substrate
• UniProt Protein Name: Hepatocyte growth factor-regulated tyrosine kinase substrate
• Protein Family: Hepatocyte growth factor-regulated tyrosine kinase substrate
• UniProt Gene Name: Hgs
• UniProt Synonym Gene Names: Hrs
• UniProt Entry Name: HGS_MOUSE

Uniprot Description

Hrs: a zinc finger protein which is rapidly tyrosine phosphorylated in cells stimulated with various growth factors. Hrs mRNA is expressed in all the human adult and fetal tissues tested.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Cellular Component: membrane; intracellular membrane-bound organelle; early endosome; cytoplasm; cytosol; endosome; secretory granule

Molecular Function: protein domain specific binding; metal ion binding

Biological Process: intracellular protein transport; protein targeting to lysosome; protein transport; transport; membrane invagination; endosome to lysosome transport; negative regulation of JAK-STAT cascade