Rabbit RGS19 Polyclonal Antibody | anti-RGS19 antibody

RGS19 (Phospho-S151) Polyclonal Antibody

Cat. Num. AAA3007908

• Gene Names: RGS19; GAIP; RGSGAIP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RGS19; Polyclonal Antibody; RGS19 (Phospho-S151) Polyclonal Antibody; Regulator of G-protein signaling 19; G-alpha-interacting protein; GAIP; GNAI3IP; anti-RGS19 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RGS19 (phospho-S151) polyclonal antibody detects endogenous levels of RGS19 protein only when phosphorylated at Ser151.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 217

• Applicable Applications for anti-RGS19 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human RGS19 around the phosphorylation site of Serine 151.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RGS19 (phospho-S151) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug))

Related Product Information for anti-RGS19 antibody

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four Galpha GTPase-activating proteins (GAPs) have been identified and are designated RGS1 (regulator of G protein signaling), RGS4, RGS10 and GAIP (Galpha-interacting protein). Each of these proteins has been shown to deactivate specific Galpha isoforms by increasing the rate at which they convert GTP to GDP, RGS1, RGS4 and GAIP bind tightly to and exhibit GAP activity towards Galpha i, Galpha o and Galpha t, but not Galpha s. RGS10 increases the GTP hydrolytic activity of several members of the Galpha i subfamily including Galpha i-3, Galpha z and Galpha o.

NCBI and Uniprot Product Information

• NCBI GI #: 86990435
• NCBI GeneID: 10287
• NCBI Accession #: NP_001034556.1
• NCBI GenBank Nucleotide #: NP_001034556.1
• UniProt Accession #: P49795
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: regulator of G-protein signaling 19
• NCBI Official Synonym Full Names: regulator of G protein signaling 19
• NCBI Official Symbol: RGS19
• NCBI Official Synonym Symbols: GAIP; RGSGAIP
• NCBI Protein Information: regulator of G-protein signaling 19
• UniProt Protein Name: Regulator of G-protein signaling 19
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS19
• UniProt Synonym Gene Names: GAIP; GNAI3IP; RGS19; GAIP
• UniProt Entry Name: RGS19_HUMAN

NCBI Description

G proteins mediate a number of cellular processes. The protein encoded by this gene belongs to the RGS (regulators of G-protein signaling) family and specifically interacts with G protein, GAI3. This protein is a guanosine triphosphatase-activating protein that functions to down-regulate Galpha i/Galpha q-linked signaling. Alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS19: inhibits signal transduction by increasing the GTPase activity of g protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(I)a3 > G(I)a1 > G(O)a >> G(Z)a/G(I)a2. Activity on G(Z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: Golgi apparatus; clathrin-coated vesicle; membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; brush border

Molecular Function: protein binding; G-protein alpha-subunit binding; GTPase activator activity

Biological Process: G-protein coupled receptor protein signaling pathway; small GTPase mediated signal transduction; autophagy; positive regulation of GTPase activity