Goat anti-Human Restrictin Polyclonal Antibody | anti-TNR antibody

Restrictin (Janusin, Tenascin R)

Cat. Num. AAA614768

• Gene Names: TNR; TNR
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by human Tenascin R affinity chromatography.
• Synonyms: Restrictin; Polyclonal Antibody; Restrictin (Janusin; Tenascin R); Anti -Restrictin (Janusin; anti-TNR antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Selected for its ability to recognize human Tenascin R in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 1% crossreactivity with rhTenascin C.
• Purity/Purification: Affinity Purified; Purified by human Tenascin R affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

• Applicable Applications for anti-TNR antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Tenascin R. The detection limit for rhTenascin R is approximately 2 ng/lane and 20 ng/lane under non-reducing and reducing conditions, respectively., Immunohistochemistry: This antibody will detect human Tenascin R in cells and tissues. The working dilution is 2-5ug/ml., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Tenascin R. The detection limit for rhTenascin R is approximately 0.3 ng/well., Optimal dilutions should be determined by each laboratory for each application.
• Immunogen: NS0-derved rhTenascin R isoform 1
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Tenascin R in Human Brainstem. Tenascin R was detected in immersion fixed paraffin-embedded sections of human brainstem (medulla) using 1.7ug/ml MBS614768 overnight at 4°C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).)

Related Product Information for anti-TNR antibody

Tenascin R is a 180 kD, 1327 amino acid, extracellular matrix glycoprotein belonging to the Tenascin family of adhesion proteins. Tenascin R is selectively expressed in the central nervous system, affecting neurite outgrowth and synaptic functions. Tenascin R isoform 2 shows a deletion of fibronectin type III repeat #6 (aa # 773-862). The human Tenascin R isoform 1 amino acid sequence is 94% identical to the corresponding canine and mouse sequences.

Product Categories/Family for anti-TNR antibody

Antibodies; Abs to Extracellular Matrix Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 63614
• NCBI GeneID: 396213
• UniProt Accession #: Q00546
• Molecular Weight: 148,280 Da
• NCBI Official Full Name: restrictin-precursor
• NCBI Official Synonym Full Names: tenascin R (restrictin, janusin)
• NCBI Official Symbol: TNR
• NCBI Official Synonym Symbols: TNR
• NCBI Protein Information: tenascin-R; TN-R; restrictin
• UniProt Protein Name: Tenascin-R
• Protein Family: Tenascin
• UniProt Gene Name: TNR
• UniProt Entry Name: TENR_CHICK

Uniprot Description

Function: Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. Involved in cell attachment and neurite formation. Interaction with CNTN1 enhances the neurite outgrowth. Ref.2

Subunit structure: Forms homodimers and homotrimers. Interacts with CNTN1, NFASC and CSPG5. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5

Subcellular location: Secreted › extracellular space › extracellular matrix. Note: May be attach to the cell surface of neural cells.

Tissue specificity: Brain specific. Ref.1

Developmental stage: Expression weakly detectable at E6 embryo, reaches a maximum at E16 and declines in the adult. Ref.1

Domain: The N-terminus cysteine-rich segment may mediate the formation of oligomers. The fibronectin type-III 2-3 mediate the binding to contactin 1. The fibronectin type-III 9 mediates the cell attachment. The fibronectin type-III 2-5 mediate NFASC binding. The fibrinogen C-terminal domain mediates interaction with CSPG5. Ref.5

Sequence similarities: Belongs to the tenascin family.Contains 5 EGF-like domains.Contains 1 fibrinogen C-terminal domain.Contains 9 fibronectin type-III domains.