Rat Neurogenic locus notch homolog protein 2 ELISA Kit | Notch2 elisa kit

Rat Neurogenic locus notch homolog protein 2 ELISA Kit

Cat. Num. AAA762017

• Reactivity: Rat
• Synonyms: Neurogenic locus notch homolog protein 2; Rat Neurogenic locus notch homolog protein 2 ELISA Kit; Notch2/AGS2/hN2/neurogenic locus notch homolog protein 2/Notch (Drosophila) homolog 2/notch 2Notch homolog 2 (Drosophila)/Notch homolog 2; Notch2 elisa kit

• Reactivity: Rat
• Sequence Length: 2471

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Notch2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Notch2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Notch2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Notch2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Notch2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 767172165
• NCBI GeneID: 29492
• NCBI Accession #: NP_077334.2
• NCBI GenBank Nucleotide #: NM_024358.2
• Molecular Weight: 265,370 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 2
• NCBI Official Synonym Full Names: notch 2
• NCBI Official Symbol: Notch2
• NCBI Protein Information: neurogenic locus notch homolog protein 2
• UniProt Protein Name: Neurogenic locus notch homolog protein 2
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: Notch2
• UniProt Synonym Gene Names: Notch 2
• UniProt Entry Name: NOTC2_RAT

NCBI Description

transmembrane protein that may be involved in cellular interactions that specify cell fate [RGD, Feb 2006]

Uniprot Description

Notch 2: a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. Affects the implementation of differentiation, proliferation and apoptotic programs. Cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for the membrane bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.

Cellular Component: cell surface; cilium; cytosol; extracellular region; integral to plasma membrane; membrane; nucleoplasm; nucleus; plasma membrane; receptor complex

Molecular Function: calcium ion binding; enzyme binding; NF-kappaB binding; receptor activity

Biological Process: bone remodeling; cell cycle arrest; cell fate determination; cell growth; defense response to bacterium; determination of left/right symmetry; embryonic limb morphogenesis; heart looping; humoral immune response; in utero embryonic development; inflammatory response to antigenic stimulus; liver development; marginal zone B cell differentiation; morphogenesis of an epithelial sheet; multicellular organism growth; myeloid dendritic cell differentiation; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; Notch signaling pathway; organ morphogenesis; placenta development; positive regulation of apoptosis; positive regulation of BMP signaling pathway; positive regulation of cell proliferation; positive regulation of osteoclast differentiation; positive regulation of Ras protein signal transduction; regulation of developmental process; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; tissue regeneration; transcription, DNA-dependent; wound healing