Western Blot (WB)
(Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Human HSPA8 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Heat Shock 70kDa Protein 8 (HSPA8) Polyclonal Antibody | anti-HSPA8 antibody
Polyclonal Antibody to Heat Shock 70kDa Protein 8 (HSPA8)
MGHHHHHHSGS-DKVSSKN SLESYAFNMK ATVEDEKLQG KINDEDKQKI LDKCNEIINW LDKNQTAEKE EFEHQQKELE KVCNPIITKL YQSAG
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Human HSPA8 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Human HSPA8 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Chaperone; Heat shock protein; Nucleolus; RNA-binding
Chromosomal Location of Human Ortholog: 11q24.1
Cellular Component: blood microparticle; cytosol; extracellular exosome; extracellular matrix; extracellular region; extracellular space; ficolin-1-rich granule lumen; focal adhesion; intracellular; intracellular ribonucleoprotein complex; late endosome; lysosomal lumen; lysosomal membrane; melanosome; membrane; myelin sheath; nucleolus; nucleoplasm; nucleus; plasma membrane; presynapse; secretory granule lumen; spliceosomal complex; ubiquitin ligase complex
Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; cadherin binding; enzyme binding; G-protein coupled receptor binding; heat shock protein binding; phosphatidylserine binding; protein binding; RNA binding; ubiquitin protein ligase binding; unfolded protein binding
Biological Process: ATP metabolic process; cellular response to starvation; chaperone cofactor-dependent protein folding; chaperone-mediated autophagy translocation complex disassembly; chaperone-mediated protein transport involved in chaperone-mediated autophagy; clathrin coat disassembly; late endosomal microautophagy; mRNA splicing, via spliceosome; negative regulation of transcription, DNA-dependent; neurotransmitter secretion; neutrophil degranulation; positive regulation of mRNA splicing, via spliceosome; protein amino acid methylation; protein folding; protein refolding; protein targeting to lysosome involved in chaperone-mediated autophagy; regulation of cell cycle; regulation of cellular response to heat; regulation of mRNA stability; regulation of protein complex assembly; regulation of protein import; regulation of protein stability; response to unfolded protein; transcription, DNA-dependent; viral process
Research Articles on HSPA8
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Product Notes
The HSPA8 hspa8 (Catalog #AAA2002024) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heat Shock 70kDa Protein 8 (HSPA8) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock 70kDa Protein 8 (HSPA8) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HSPA8 hspa8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S-DKVSSKN SLESYAFNMK ATVEDEKLQG KINDEDKQKI LDKCNEIINW LDKNQTAEKE EFEHQQKELE KVCNPIITKL YQSAG. It is sometimes possible for the material contained within the vial of "Heat Shock 70kDa Protein 8 (HSPA8), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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