Western Blot (WB)
(Western blot analysis of phosphorylated HDAC-7 in (A) recombinant fusion protein containing a phosphorylated serine at position 155 and (B) fusion protein containing an unphosphorylated serine at position 155, using MBS647965 at 0.1ug/ml.)
Rabbit HDAC7 Polyclonal Antibody | anti-HDAC7 antibody
HDAC7, phosphorylated (Ser155) (Histone Deacetylase 7, HD7, DKFZP586J0917, HD7a, Histone Deacetylase 7A, HDAC7A)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 0.1-1ug/ml
Western Blot (WB)
(Western blot analysis of phosphorylated HDAC-7 in (A) recombinant fusion protein containing a phosphorylated serine at position 155 and (B) fusion protein containing an unphosphorylated serine at position 155, using MBS647965 at 0.1ug/ml.)
Western Blot (WB)
(Western blot analysis of phosphorylated HDAC-7 in (A) recombinant fusion protein containing a phosphorylated serine at position 155 and (B) fusion protein containing an unphosphorylated serine at position 155, using MBS647965 at 0.1ug/ml.)
NCBI and Uniprot Product Information
NCBI Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors
By similarity. May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. Ref.12
Catalytic activity: Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.
Subunit structure: Interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3. Interacts with the 14-3-3 protein YWHAE, MEF2A, MEF2B and MEF2C
By similarity. Interacts with KAT5 and EDNRA. Interacts with KDM5B. Interacts with ZMYND15
By similarity. Interacts with PML (isoform PML-4). Ref.10 Ref.11 Ref.13 Ref.16 Ref.23
Subcellular location: Nucleus. Cytoplasm. Note: In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and is due to its phosphorylation. Ref.10 Ref.14
Domain: The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm
By similarity.
Post-translational modification: May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylation at Ser-155 by MARK2, MARK3 and PRKD1 promotes interaction with 14-3-3 proteins and export from the nucleus. Phosphorylation at Ser-155 is a prerequisite for phosphorylation at Ser-181. Ref.14 Ref.15 Ref.20
Miscellaneous: Its activity is inhibited by Trichostatin A (TSA), a known histone deacetylase inhibitor
By similarity.
Sequence similarities: Belongs to the histone deacetylase family. HD type 2 subfamily.
Sequence caution: The sequence AAF63491.1 differs from that shown. Reason: Frameshift at position 877. The sequence BAA91474.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA91545.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15759.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB55363.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC56929.1 differs from that shown. Reason: Intron retention.
Research Articles on HDAC7
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Product Notes
The HDAC7 hdac7 (Catalog #AAA647965) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HDAC7, phosphorylated (Ser155) (Histone Deacetylase 7, HD7, DKFZP586J0917, HD7a, Histone Deacetylase 7A, HDAC7A) reacts with Bovine, Canine, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 0.1-1ug/ml. Researchers should empirically determine the suitability of the HDAC7 hdac7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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