Rabbit anti-Human HDAC7 Polyclonal Antibody | anti-HDAC7 antibody

HDAC7, CT (Histone Deacetylase 7, HD7, Histone Deacetylase 7A, HD7a, HDAC7A)

Cat. Num. AAA628659

• Gene Names: HDAC7; HD7A; HDAC7A; FLJ99588; DKFZp586J0917
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: HDAC7; Polyclonal Antibody; CT (Histone Deacetylase 7; HD7; Histone Deacetylase 7A; HD7a; HDAC7A); Anti -HDAC7; anti-HDAC7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HDAC7.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-HDAC7 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human HDAC7 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hHDAC7-A935 in CEM cell line lysates (35ug/lane). HDAC7 (arrow) was detected using the purified Pab.)

Related Product Information for anti-HDAC7 antibody

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Product Categories/Family for anti-HDAC7 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 148539870
• NCBI GeneID: 51564
• NCBI Accession #: NP_001091886.1
• NCBI GenBank Nucleotide #: NM_001098416.2
• UniProt Accession #: Q8WUI4; Q6P1W9; Q6W9G7; Q7Z4K2; Q7Z5I1; Q96K01; Q9BR73; Q9H7L0; Q9NW41; B3KY08; B4DWI0; B4E0Q5; Q9H5S4
• Molecular Weight: 102,927 Da
• NCBI Official Full Name: histone deacetylase 7 isoform d
• NCBI Official Synonym Full Names: histone deacetylase 7
• NCBI Official Symbol: HDAC7
• NCBI Official Synonym Symbols: HD7A; HDAC7A; FLJ99588; DKFZp586J0917
• NCBI Protein Information: histone deacetylase 7; HD7; OTTHUMP00000202813; OTTHUMP00000202814; histone deacetylase 7A
• UniProt Protein Name: Histone deacetylase 7
• UniProt Gene Name: HDAC7
• UniProt Synonym Gene Names: HDAC7A
• UniProt Entry Name: HDAC7_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

HDAC7: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. Interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3. Interacts with the 14-3-3 protein YWHAE, MEF2A, MEF2B and MEF2C. Interacts with KAT5 and EDNRA. Interacts with KDM5B. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: nucleoplasm; histone deacetylase complex; cytoplasm; nucleus

Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; transcription activator binding; protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; chromatin binding; NAD-dependent histone deacetylase activity (H4-K16 specific); transcription corepressor activity; protein kinase binding

Biological Process: intercellular junction assembly; Notch signaling pathway; transcription, DNA-dependent; negative regulation of osteoblast differentiation; negative regulation of transcription from RNA polymerase II promoter; vasculogenesis; negative regulation of interleukin-2 production