Rabbit ATG4B Polyclonal Antibody | anti-ATG4B antibody

ATG4B Antibody

Cat. Num. AAA153439

• Gene Names: ATG4B; APG4B; AUTL1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: ATG4B antibody is affinity chromatography purified via peptide column.
• Synonyms: ATG4B; Polyclonal Antibody; ATG4B Antibody; Autophagy related 4B cysteine peptidase; APG4B; APG4-B; AUTL1; autophagy related 4B; cysteine peptidase; anti-ATG4B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATG4B antibody is human, mouse and rat reactive. At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.
• Purity/Purification: ATG4B antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-ATG4B antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: ATG4B antibody can be used for detection of ATG4B by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B.
• Buffer: ATG4B antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ATG4B antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 μg/ml.)

Related Product Information for anti-ATG4B antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4B, also known as AUTL1, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4). ATG4B has been found to be a novel protective protein in inflammatory colitis (5).

NCBI and Uniprot Product Information

• NCBI GI #: 47132611
• NCBI GeneID: 23192
• NCBI Accession #: NP_037457
• NCBI GenBank Nucleotide #: NM_013325.4
• UniProt Accession #: Q9Y4P1; Q53NU4; Q6ZUV8; Q8WYM9; Q96K07; Q96K96; Q96SZ1; Q9Y2F2; B7WNK2
• Molecular Weight: Predicted: 43 kDa; ; Observed: 44 kDa
• NCBI Official Full Name: cysteine protease ATG4B isoform a
• NCBI Official Synonym Full Names: autophagy related 4B, cysteine peptidase
• NCBI Official Symbol: ATG4B
• NCBI Official Synonym Symbols: APG4B; AUTL1
• NCBI Protein Information: cysteine protease ATG4B; hAPG4B; autophagin-1; APG4 autophagy 4 homolog B; AUT-like 1 cysteine endopeptidase; ATG4 autophagy related 4 homolog B; autophagy-related protein 4 homolog B; autophagy-related cysteine endopeptidase 1
• UniProt Protein Name: Cysteine protease ATG4B
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4B
• UniProt Synonym Gene Names: APG4B; AUTL1; KIAA0943; hAPG4B
• UniProt Entry Name: ATG4B_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4B: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Autophagy; Ubiquitin conjugating system; EC 3.4.22.-

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: protein delipidation; positive regulation of protein catabolic process; mitochondrion degradation; autophagy; C-terminal protein lipidation; protein processing; proteolysis; protein targeting to membrane; positive regulation of autophagy; cellular response to nitrogen starvation; autophagic vacuole formation