Rabbit HDAC6 Polyclonal Antibody | anti-HDAC6 antibody

HDAC6 Antibody

Cat. Num. AAA2528538

• Gene Names: HDAC6; CG6170; dHDAC2; DHDAC2; dHDAC6; DmelCG6170; dmHDA404; DmHDAC2; HDAC; HDAC2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antigen affinity purification
• Synonyms: HDAC6; Polyclonal Antibody; HDAC6 Antibody; FLJ16239; HD6; JM21; anti-HDAC6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1179

• Applicable Applications for anti-HDAC6 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Recombinant protein of human HDAC6
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-5255 / sc-5258 / sc-11420
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HDAC6 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using HDAC6 antibody at dilution of 1:200 (x400 lens))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using HDAC6 antibody at dilution of 1:200 (x400 lens))

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using HDAC6 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-HDAC6 antibody

HDAC6 is a class II histone deacetylase enzyme localized to the cytoplasm and associated with the microtubule network (1). It is involved in the regulation of many cellular processes, including cell migration, immune synapse formation, viral infection, and degradation of misfolded proteins (1). HDAC6 contains two tandem catalytic domains that facilitate the deacetylation of multiple protein substrates, including histones and non-histone proteins such as tubulin, cortactin, and HSP90. Despite the ability to deacetylate histone proteins in vitro, there is no evidence for HDAC6-mediated deacetylation of histones in vivo (2, 3). The acetylation/deacetylation of tubulin on Lys40 regulates binding and motility of the kinesin-1 motor protein and subsequent transport of cargo proteins such as JNK-interacting protein 1 (JIP1) (4). The acetylation/deacetylation of cortactin regulates cell motility by modulating the binding of cortactin to F-actin (5). Acetylation/deacetylation of HSP90 modulates chaperone complex activity by regulating the binding of an essential cochaperone protein, p23 (6, 7). In addition to its role as a protein deacetylase, HDAC6 functions as a component of the aggresome, a proteinaceous inclusion body that forms in response to an accumulation of misfolded or partially denatured proteins (8). Formation of the aggresome is a protective response that sequesters cytotoxic protein aggregates for eventual autophagic clearance from the cell. HDAC6 contains a zinc finger ubiquitin-binding domain that binds both mono- and poly-ubiquitinated proteins (8).

NCBI and Uniprot Product Information

• NCBI GI #: 442616414
• NCBI GeneID: 32461
• NCBI Accession #: NP_001259569.1
• NCBI GenBank Nucleotide #: NM_001272640.1
• Molecular Weight: 124,871 Da
• NCBI Official Full Name: HDAC6, isoform G
• NCBI Official Symbol: HDAC6
• NCBI Official Synonym Symbols: CG6170; dHDAC2; DHDAC2; dHDAC6; DmelCG6170; dmHDA404; DmHDAC2; HDAC; HDAC2
• NCBI Protein Information: CG6170 gene product from transcript CG6170-RA; CG6170-PA; CG6170-PB; CG6170-PC; CG6170-PD; CG6170-PE; CG6170-PF; CG6170-PG; HDAC6; HDAC6-PA; HDAC6-PB; HDAC6-PC; HDAC6-PD; HDAC6-PE; HDAC6-PF; HDAC6-PG; histone deacetylase 6; histone deacetylase HDA2
• UniProt Protein Name: HDAC6, isoform A
• UniProt Gene Name: HDAC6
• UniProt Entry Name: Q86NK9_DROME