Rabbit HDAC6 Polyclonal Antibody | anti-HDAC6 antibody

Anti-HDAC6 Antibody

Cat. Num. AAA419790

• Gene Names: HDAC6; HD6; JM21; CPBHM; PPP1R90
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: HDAC6; Polyclonal Antibody; Anti-HDAC6 Antibody; anti-HDAC6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using HDAC6 antibody. Immunofluorescence analysis of U2OS cell using HDAC6 antibody. Blue: DAPI for nuclear staining.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1215

• Applicable Applications for anti-HDAC6 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:50- 1:200
• Immunogen: Recombinant protein of human HDAC6
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunofluorescence (IF)

Related Product Information for anti-HDAC6 antibody

HDAC6 is a class II histone deacetylase enzyme localized to the cytoplasm and associated with the microtubule network (1). It is involved in the regulation of many cellular processes, including cell migration, immune synapse formation, viral infection, and degradation of misfolded proteins (1). HDAC6 contains two tandem catalytic domains that facilitate the deacetylation of multiple protein substrates, including histones and non-histone proteins such as tubulin, cortactin, and HSP90. Despite the ability to deacetylate histone proteins in vitro, there is no evidence for HDAC6-mediated deacetylation of histones in vivo (2, 3). The acetylation/deacetylation of tubulin on Lys40 regulates binding and motility of the kinesin-1 motor protein and subsequent transport of cargo proteins such as JNK-interacting protein 1 (JIP1) (4). The acetylation/deacetylation of cortactin regulates cell motility by modulating the binding of cortactin to F-actin (5). Acetylation/deacetylation of HSP90 modulates chaperone complex activity by regulating the binding of an essential cochaperone protein, p23 (6, 7). In addition to its role as a protein deacetylase, HDAC6 functions as a component of the aggresome, a proteinaceous inclusion body that forms in response to an accumulation of misfolded or partially denatured proteins (8). Formation of the aggresome is a protective response that sequesters cytotoxic protein aggregates for eventual autophagic clearance from the cell. HDAC6 contains a zinc finger ubiquitin-binding domain that binds both mono- and poly-ubiquitinated proteins (8). HDAC6 binds to both poly-ubiquitinated misfolded proteins and dynein motors, facilitating the transport of misfolded proteins to the aggresome (9, 10). HDAC6 is also required for subsequent recruitment of the autophagic machinery and clearance of aggresomes from the cell (11). Thus, HDAC6 plays a key role in the protection against the deleterious effects of pathological protein aggregation that occurs in various diseases, such as neurodegenerative Huntington

NCBI and Uniprot Product Information

• NCBI GI #: 46623327
• NCBI GeneID: 10013
• Molecular Weight: 114,361 Da
• NCBI Official Full Name: HDAC6 protein
• NCBI Official Synonym Full Names: histone deacetylase 6
• NCBI Official Symbol: HDAC6
• NCBI Official Synonym Symbols: HD6; JM21; CPBHM; PPP1R90
• NCBI Protein Information: histone deacetylase 6
• UniProt Protein Name: Histone deacetylase 6
• UniProt Gene Name: HDAC6
• UniProt Synonym Gene Names: KIAA0901; HD6

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC6: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily.

Protein type: Deacetylase; EC 3.5.1.98; Nuclear receptor co-regulator; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: axon; caveola; cytoplasm; cytosol; dendrite; dynein complex; histone deacetylase complex; inclusion body; leading edge; microtubule; microtubule associated complex; multivesicular body; nucleus; perikaryon; perinuclear region of cytoplasm

Molecular Function: alpha-tubulin binding; beta-catenin binding; enzyme binding; histone deacetylase activity; histone deacetylase binding; Hsp90 protein binding; microtubule binding; misfolded protein binding; polyubiquitin binding; protein binding; tau protein binding; tubulin deacetylase activity; ubiquitin protein ligase binding

Biological Process: histone deacetylation; intracellular protein transport; lysosome localization; macroautophagy; misfolded or incompletely synthesized protein catabolic process; negative regulation of oxidoreductase activity; negative regulation of protein complex disassembly; negative regulation of proteolysis; negative regulation of transcription, DNA-dependent; organelle organization and biogenesis; positive regulation of signal transduction; protein amino acid deacetylation; regulation of autophagy; regulation of gene expression, epigenetic; regulation of protein stability; regulation of receptor activity; response to misfolded protein; response to organic substance; response to toxin

Disease: Chondrodysplasia With Platyspondyly, Distinctive Brachydactyly, Hydrocephaly, And Microphthalmia