Rabbit YMEL1 Polyclonal Antibody | anti-YMEL1 antibody

YMEL1, NT (YME1L1, FTSH1, YME1L, ATP-dependent zinc metalloprotease YME1L1, ATP-dependent metalloprotease FtsH1, Meg-4, Presenilin-associated metalloprotease, YME1-like protein 1) (Azide free) (HRP)

Cat. Num. AAA6364329

• Gene Names: YME1L1; FTSH; MEG4; PAMP; YME1L
• Reactivity: Hamster, Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: YMEL1; Polyclonal Antibody; NT (YME1L1; FTSH1; YME1L; ATP-dependent zinc metalloprotease YME1L1; ATP-dependent metalloprotease FtsH1; Meg-4; Presenilin-associated metalloprotease; YME1-like protein 1) (Azide free) (HRP); YME1L1; YME1-like protein 1; anti-YMEL1 antibody

• Host: Rabbit
• Reactivity: Hamster, Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-YMEL1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: YMEL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 140-170 from the N-terminal region of human YMEL1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of YMEL1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). YMEL1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of YMEL1 Antibody (N-term) in CHO cell line lysates (35ug/lane). YMEL1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(YMEL1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YMEL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(YMEL1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-YMEL1 antibody

YMEL1 is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies.

Product Categories/Family for anti-YMEL1 antibody

Antibodies; Enzymes

References

Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Deloukas, P., et al. Nature 429(6990):375-381(2004). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 46397258
• NCBI GeneID: 10730
• UniProt Accession #: Q96TA2; Q5T8D9; Q9H1Q0; Q9UMR9; B4DNM1; D3DRV8; D3DRV9
• Molecular Weight: 75,982 Da
• NCBI Official Full Name: ATP-dependent zinc metalloprotease YME1L1
• NCBI Official Synonym Full Names: YME1 like 1 ATPase
• NCBI Official Symbol: YME1L1
• NCBI Official Synonym Symbols: FTSH; MEG4; PAMP; YME1L
• NCBI Protein Information: ATP-dependent zinc metalloprotease YME1L1
• UniProt Protein Name: ATP-dependent zinc metalloprotease YME1L1
• UniProt Gene Name: YME1L1
• UniProt Synonym Gene Names: FTSH1; YME1L; PAMP
• UniProt Entry Name: YMEL1_HUMAN

NCBI Description

The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

YME1L1: Putative ATP-dependent protease which plays a role in mitochondrial protein metabolism. Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. Requires to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1). Seems to act in the processing of OPA1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 10p14

Cellular Component: integral to membrane; membrane; mitochondrial inner membrane; mitochondrion; nucleoplasm

Molecular Function: ATP binding; ATP-dependent peptidase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity

Biological Process: cell proliferation; misfolded or incompletely synthesized protein catabolic process; mitochondrion organization and biogenesis