Western Blot (WB)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HDAC2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human HDAC2 )
Mouse anti-Human Histone Deacetylase 2/HDAC2 Monoclonal Antibody | anti-HDAC2 antibody
Histone Deacetylase 2/HDAC2
Western Blot (WB)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HDAC2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human HDAC2 )
Western Blot (WB)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HDAC2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human HDAC2 )
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of HDAC2 in A549 cells. The cell was stained at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black). )
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of HDAC2 in A549 cells. The cell was stained at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black). )
NCBI and Uniprot Product Information
NCBI Description
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
Uniprot Description
HDAC2: a transcriptional regulator of the histone deacetylase family, subfamily 1. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays a role in epigenetic repression and transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Protein type: Deacetylase; Transcription, coactivator/corepressor; EC 3.5.1.98; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: nucleoplasm; heterochromatin; transcription factor complex; protein complex; Sin3 complex; ESC/E(Z) complex; nuclear chromatin; cytoplasm; replication fork; NuRD complex; nucleus
Molecular Function: deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; nucleosomal DNA binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; heat shock protein binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription factor activity; transcription factor binding
Biological Process: establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; cardiac muscle cell development; negative regulation of gene expression, epigenetic; negative regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; dendrite development; circadian regulation of gene expression; positive regulation of oligodendrocyte differentiation; response to drug; transcription, DNA-dependent; negative regulation of transcription factor activity; hippocampus development; histone deacetylation; regulation of protein kinase B signaling cascade; response to cocaine; odontogenesis of dentine-containing teeth; chromatin remodeling; ATP-dependent chromatin remodeling; negative regulation of MHC class II biosynthetic process; maintenance of chromatin silencing; epidermal cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; gene expression; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; blood coagulation; negative regulation of apoptosis
Research Articles on HDAC2
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Product Notes
The HDAC2 hdac2 (Catalog #AAA200473) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone Deacetylase 2/HDAC2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone Deacetylase 2/HDAC2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the HDAC2 hdac2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone Deacetylase 2/HDAC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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