Rabbit anti-Human HDAC10 Polyclonal Antibody | anti-HDAC10 antibody

HDAC10, NT (Histone Deacetylase 10, HD10)

Cat. Num. AAA628694

• Gene Names: HDAC10; HD10; MGC149722; DKFZp761B039
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: HDAC10; Polyclonal Antibody; NT (Histone Deacetylase 10; HD10); Anti -HDAC10; anti-HDAC10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HDAC10.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-HDAC10 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa24-54 of human HDAC10.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-HDAC10 Pab in A375 cell line lysates. HDAC10 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of HDAC10 (arrow) using rabbit polyclonal HDAC10 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC10 gene (Lane 2) (Origene Technologies).)

Related Product Information for anti-HDAC10 antibody

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo. HDAC8 interacts with PEPB2-MYH11, a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11 produced by the inversion Inv(16)(p13q22), a translocation associated with acute myeloid leukemia of M4EO subtype. The PEPB2-MYH1 fusion protein also interacts with RUNX1, a well known transcriptional regulator, suggesting that the interaction with HDAC8 may participate to convert RUNX1 into a constitutive transcriptional repressor.

Product Categories/Family for anti-HDAC10 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 226529806
• NCBI GeneID: 83933
• NCBI Accession #: NP_001152758.1
• NCBI GenBank Nucleotide #: NM_001159286.1
• UniProt Accession #: Q08AP5; Q59FP1; Q969S8
• Molecular Weight: 11,171 Da
• NCBI Official Full Name: histone deacetylase 10 isoform 2
• NCBI Official Synonym Full Names: histone deacetylase 10
• NCBI Official Symbol: HDAC10
• NCBI Official Synonym Symbols: HD10; MGC149722; DKFZp761B039
• NCBI Protein Information: histone deacetylase 10; OTTHUMP00000042162; OTTHUMP00000196674; OTTHUMP00000196675; OTTHUMP00000197621
• UniProt Protein Name: HDAC10 protein
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC10
• UniProt Entry Name: Q08AP5_HUMAN

NCBI Description

Acetylation of histone core particles modulates chromatin structure and gene expression. The opposing enzymatic activities of histone acetyltransferases and histone deacetylases, such as HDAC10, determine the acetylation status of histone tails (Kao et al., 2002 [PubMed 11677242]).[supplied by OMIM]

Uniprot Description

HDAC10: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Belongs to the histone deacetylase family. HD type 2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; EC 3.5.1.98; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; cytoplasm; histone deacetylase complex; nucleus

Molecular Function: protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity

Biological Process: establishment and/or maintenance of chromatin architecture; Notch signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; protein amino acid deacetylation; histone deacetylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; oligodendrocyte development; negative regulation of transcription, DNA-dependent