Rabbit anti-Human HDAC10 Polyclonal Antibody | anti-HDAC10 antibody

HDAC10, NT (Histone Deacetylase 10, HD10) (PE)

Cat. Num. AAA6479596

• Gene Names: HDAC10; HD10
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: HDAC10; Polyclonal Antibody; NT (Histone Deacetylase 10; HD10) (PE); anti-HDAC10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HDAC10.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HDAC10 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa24-54 of human HDAC10.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-HDAC10 Pab in A375 cell line lysates. HDAC10(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of HDAC10 (arrow) using rabbit polyclonal HDAC10 Antibody (N-term) (RB02583). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC10 gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-HDAC10 antibody

Antibodies; Enzymes; Deacetylase

References

1. Keedy, K.S. et al. J Virol. May; 83(10): 4749-4756(2009). 2. Tong, J.J., et al., Nucleic Acids Res. 30(5):1114-1123 (2002). 3. Fischer, D.D., et al., J. Biol. Chem. 277(8):6656-6666 (2002). 4. Guardiola, A.R., et al., J. Biol. Chem. 277(5):3350-3356 (2002). 5. Kao, H.Y., et al., J. Biol. Chem. 277(1):187-193 (2002). 6.Kara S. Keedy, et al. J. Virol. 2009; 83:4749-4756. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 226529806
• NCBI GeneID: 83933
• NCBI Accession #: NP_001152758.1
• NCBI GenBank Nucleotide #: NM_001159286.1
• UniProt Accession #: Q969S8; Q08AP4; Q6STF9; Q96P77; Q96P78; Q9H028; Q9UGX1; Q9UGX2
• Molecular Weight: 71,445 Da
• NCBI Official Full Name: histone deacetylase 10 isoform 2
• NCBI Official Synonym Full Names: histone deacetylase 10
• NCBI Official Symbol: HDAC10
• NCBI Official Synonym Symbols: HD10
• NCBI Protein Information: histone deacetylase 10
• UniProt Protein Name: Histone deacetylase 10
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC10
• UniProt Synonym Gene Names: HD10
• UniProt Entry Name: HDA10_HUMAN

NCBI Description

The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

HDAC10: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Belongs to the histone deacetylase family. HD type 2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; EC 3.5.1.98; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; histone deacetylase complex; cytoplasm; nucleus

Molecular Function: protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity

Biological Process: Notch signaling pathway; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; protein amino acid deacetylation; regulation of transcription, DNA-dependent; histone deacetylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; oligodendrocyte development; negative regulation of transcription, DNA-dependent