Rabbit anti-Human HCN2 Polyclonal Antibody | anti-HCN2 antibody

HCN2 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 2)

Cat. Num. AAA616643

• Gene Names: HCN2; BCNG2; HAC-1; BCNG-2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: HCN2; Polyclonal Antibody; HCN2 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 2); Anti -HCN2 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 2); anti-HCN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HCN2. No significant sequence homology is seen with other HCNs or any other protein. Species sequence homology: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.1% sodium azide, 40% glycerol.

• Applicable Applications for anti-HCN2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
• Immunogen: Synthetic peptide, 15aa near the cytoplasmic, N-terminus of human HCN2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HCN2 antibody

Biological rhythms (beating of the heart, circadian sleep cycles, respiration, and the release of hormones) are necessary to sustain life. Heart beating is the most reliable and rhythmic biological phenomenon. Cardiac pacemaking is produced by the slow diastolic depolarization phase of the action potential. The hyperpolarization-activated cation current (termed If, Ih, or Iq) plays a key role in the in the initiation and modulation of cardiac and neuronal pacemaker depolarization. The generation of cardiac pacemaker potentials relies on a complex interplay between at least four different types of cation channels: T- and L-type Ca2+ channels, K+ channels, and a cation channel termed If (synonymous names are Ih and Iq). The If channel has been designated as "pacemaker" channel because it reveals unique features that are believed to be a prerequisite for pacemaker activity. Recently, the hyperpolarization-activated cyclic nucleotide-gated families of ion channel proteins (HCN1-4) have been identified as the "pacemaker" channel. The amino acid sequences of HCNs predict a structure similar to that of voltage-gated (Kv) channels and cyclic nucleotide-gated (CNG) channels. HCNs proteins (HCN1-4) are characterized by six transmembrane domains (S1-S6), including a including a positively charged voltage-sensing S4 segment and an ion-conducting pore between S5 and S6. In the C terminus the HCNs carry a cyclic nucleotide-binding domain (CNBD), a motif found in several cyclic nucleotide-binding proteins. The core region of HCNs channels (S1 to the C terminus of the CNBD) is highly conserved, whereas the cytoplasmic N and C-termini, vary considerably in their length and share only weak sequence homology. The functional HCN, like other members of the superfamily of voltage-gated cation channels, channels probably assemble into tetrameric complexes.

HCN2, also known as Brain cyclic nucleotide channel gated 2, BCNG2 or HAC-1 (rat 834 aa, human 889-aa, chromosome 19p13.3), is primarily expressed in brain. In mouse, HCN1 is mainly expressed in brain (olfactory bulb and cerebral cortex, hippocampus, thalamus, cerebellum etc) and heart (ventricles and atrium).
HCN2, also known as Brain cyclic nucleotide channel gated 2, BCNG2 or HAC-1 (rat 834 aa, human 889-aa, chromosome 19p13.3), is primarily expressed in brain. In mouse, HCN1 is mainly expressed in brain (olfactory bulb and cerebral cortex, hippocampus, thalamus, cerebellum etc) and heart (ventricles and atrium).

Product Categories/Family for anti-HCN2 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 4996894
• NCBI GeneID: 610
• UniProt Accession #: Q9UL51; O60742; O60743; O75267; Q9UBS2; Q09ND3
• Molecular Weight: 96,950 Da
• NCBI Official Full Name: hyperpolarization-activated, cyclic nucleotide-gated channel 2
• NCBI Official Synonym Full Names: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• NCBI Official Symbol: HCN2
• NCBI Official Synonym Symbols: BCNG2; HAC-1; BCNG-2
• NCBI Protein Information: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; brain cyclic nucleotide gated channel 2; brain cyclic nucleotide-gated channel 2
• UniProt Protein Name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
• Protein Family: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel
• UniProt Gene Name: HCN2
• UniProt Synonym Gene Names: BCNG2
• UniProt Entry Name: HCN2_HUMAN

NCBI Description

Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM]

Uniprot Description

HCN2: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. Belongs to the potassium channel HCN family.

Protein type: Channel, cation; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: voltage-gated potassium channel complex; integral to plasma membrane; plasma membrane

Molecular Function: identical protein binding; voltage-gated potassium channel activity; intracellular cAMP activated cation channel activity; voltage-gated sodium channel activity; cAMP binding

Biological Process: synaptic transmission; regulation of membrane potential; cell-cell signaling