Human Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit | TRPV1 elisa kit

Human Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit

Cat. Num. AAA2019906

• Gene Names: TRPV1; VR1
• Reactivity: Human
• Synonyms: Transient Receptor Potential Cation Channel Subfamily V; Member 1 (TRPV1); Human Transient Receptor Potential Cation Channel Subfamily V; Member 1 (TRPV1) ELISA Kit; VR1; OTRPC1; Vanilloid Receptor Subtype 1; Capsaicin receptor; Osm-9-like TRP channel 1; Vanilloid receptor 1; TRPV1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TRPV1. No significant cross-reactivity or interference between TRPV1 and analogues was observed.
• Sequence Length: 839

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.121ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TRPV1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TRPV1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRPV1 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TRPV1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TRPV1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TRPV1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TRPV1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TRPV1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 74315354
• NCBI GeneID: 7442
• NCBI Accession #: NP_542437.2
• NCBI GenBank Nucleotide #: NM_080706.3
• UniProt Accession #: Q8NER1; Q3LU47; Q9H0G9; Q9H303; Q9H304; Q9NQ74; Q9NY22; A2RUA9
• Molecular Weight: 94,211 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily V member 1
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily V, member 1
• NCBI Official Symbol: TRPV1
• NCBI Official Synonym Symbols: VR1
• NCBI Protein Information: transient receptor potential cation channel subfamily V member 1
• UniProt Protein Name: Transient receptor potential cation channel subfamily V member 1
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPV1
• UniProt Synonym Gene Names: VR1; TrpV1; OTRPC1
• UniProt Entry Name: TRPV1_HUMAN

NCBI Description

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRPV1: a receptor for capsaicin and a non-selective cation channel that is structurally related to members of the TRP family of ion channels. Activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo.

Protein type: Membrane protein, multi-pass; Receptor, misc.; Membrane protein, integral; Channel, cation

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; integral to membrane; cell junction; intrinsic to plasma membrane

Molecular Function: calmodulin binding; transmembrane receptor activity; excitatory extracellular ligand-gated ion channel activity; calcium-release channel activity; calcium channel activity; phosphoprotein binding; ATP binding

Biological Process: cell surface receptor linked signal transduction; transport; chemosensory behavior; thermoception; transmembrane transport