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Sequence
Sirtuin 3 (SIRT3) Active Protein | SIRT3 active protein
Active Sirtuin 3 (SIRT3)
Gene Names
Sirt3; Sir2l3; AI848213; 2310003L23Rik
Reactivity
Mus musculus (Mouse)
Applications
Cell culture; Activity Assays.
Purity
>92%
Synonyms
Sirtuin 3 (SIRT3); Active Sirtuin 3 (SIRT3); SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3; mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3; SIRT3 active protein
Host
E. coli
Reactivity
Mus musculus (Mouse)
Isotype
N.A
Specificity
N.A
Purity/Purification
>92%
Form/Format
100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5%Trehalose and Proclin300.
Sequence Positions
N.A
Sequence
N.A
Sequence Length
N.A
Applicable Applications for SIRT3 active protein
Cell culture; Activity Assays.
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression.
Residues
Gly5~Arg257
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
6.4
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Activity Data
(Figure.The binding activity of SIRT3 with DLAT.)
Activity Data
(Figure.The binding activity of SIRT3 with DLAT.)
SDS-Page
SDS-Page
Testing Data
Testing Data
Related Product Information for SIRT3 active protein
Activity: Sirtuin 3 (SIRT3) also known as NAD-dependent deacetylase sirtuin-3 is a member of the mammalian sirtuin family of proteins. SIRT3 is a soluble protein located in the mitochondrial matrix, and contains a mitochondrial processing peptide at the N-terminus. It is a key regulator of succinate dehydrogenase (SDH), which catalyzes the oxidation of succinate to fumarate. Increased succinate concentrations and the specific G protein-coupled receptor 91 (GPR91) are involved in the activation of hepatic stellate cells (HSCs). SIRT3 agonists could help treat metabolic disorders such as obesity, metabolic syndrome and type 2 diabetes. Besides, Dihydrolipoyl Transacetylase (DLAT) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SIRT3 and recombinant mouse DLAT. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to DLAT-coated microtiter wells and incubated for 2h at 37?. Wells were washed with PBST and incubated for 1h with anti-SITR3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37?. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and DLAT was shown in Figure 1, and this effect was in a dose dependent manner.
Product Categories/Family for SIRT3 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 32.2kDa
Accurate Molecular Mass: 16&29kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 16&29kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-3 isoform 1
NCBI Official Synonym Full Names
sirtuin 3
NCBI Official Symbol
Sirt3
NCBI Official Synonym Symbols
Sir2l3; AI848213; 2310003L23Rik
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-3
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-3
Protein Family
UniProt Gene Name
Sirt3
UniProt Synonym Gene Names
Sir2l3; mSIR2L3
Uniprot Description
SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product.
Protein type: Deacetylase; EC 3.5.1.-; Mitochondrial
Chromosomal Location of Human Ortholog: 7|7 F4-F5
Cellular Component: cytoplasm; membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrion
Molecular Function: enzyme binding; hydrolase activity; metal ion binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding; zinc ion binding
Biological Process: aerobic respiration; positive regulation of insulin secretion; protein deacetylation
Research Articles on SIRT3
Similar Products
Product Notes
The SIRT3 sirt3 (Catalog #AAA2097315) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is N.A. The Active Sirtuin 3 (SIRT3) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Sirtuin 3 (SIRT3) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the SIRT3 sirt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: N.A. It is sometimes possible for the material contained within the vial of "Sirtuin 3 (SIRT3), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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