Western Blot (WB)
(Western Blot (WB) analysis of HCCA2 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:Mouse liver tissue lysateLane3:Rat testis tissue lysate)
Rabbit HCCA2 Polyclonal Antibody | anti-HCCA2 antibody
HCCA2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
HCCA2; Polyclonal Antibody; HCCA2 Polyclonal Antibody; MOB kinase activator 2; Mob2 homolog; Mps one binder kinase activator-like 2; MOB2; anti-HCCA2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HCCA2 polyclonal antibody detects endogenous levels of HCCA2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
268
Applicable Applications for anti-HCCA2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human HCCA2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of HCCA2 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:Mouse liver tissue lysateLane3:Rat testis tissue lysate)
Western Blot (WB)
(Western Blot (WB) analysis of HCCA2 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:Mouse liver tissue lysateLane3:Rat testis tissue lysate)
Related Product Information for anti-HCCA2 antibody
HCCA2 (Hepatocellular carcinoma-associated gene 2), also known as MOB2 (Mps one binder kinase activator-like 2), is a 467 amino acid protein that belongs to the MOB1/phocein family. HCCA2 is expressed in lung, spleen, brain, and fetal liver. It is highly expressed in hepatocellular carcinoma. HCCA2 is localized in the perinuclear region of the cytoplasm in liver cancer tissues and colocalizes with MAD2L2 in the nucleus of Hela cells. HCCA2 is chracaterized by two N-glycosylation sites, six N-myristolylation sites, two Src homolgy 3 (SH3), and several phosphorylation motifs which indicate that this protein may play a role in an intracellular signal transduction cascade. HCCA2 binds to and regulates the autophosphorylation of the related human serine/threonine kinase 38 (NDR1) and serine/threonine kinase 38L (NDR2). It has been shown that HCCA2 plays a critical role in cell cycle regulation. Overexpression of the protein during the G0/G1 phase inhibits cell proliferation causing cell cycle arrest.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 28kDa
NCBI Official Full Name
MOB kinase activator 2 isoform 1
UniProt Protein Name
MOB kinase activator 2
UniProt Gene Name
MOB2
UniProt Synonym Gene Names
HCCA2
UniProt Entry Name
MOB2_HUMAN
Uniprot Description
HCCA2: Stimulates the autophosphorylation and kinase activity of STK38 and STK38L. Belongs to the MOB1/phocein family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleolus; nucleus
Molecular Function: metal ion binding
Biological Process: positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis
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Product Notes
The HCCA2 mob2 (Catalog #AAA3006346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HCCA2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HCCA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the HCCA2 mob2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCCA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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