Rabbit anti-Human, Mouse HADHA Polyclonal Antibody | anti-HADHA antibody

HADHA Polyclonal Antibody

Cat. Num. AAA3011143

• Gene Names: HADHA; GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HADHA; Polyclonal Antibody; HADHA Polyclonal Antibody; Trifunctional enzyme subunit alpha; mitochondrial; 78 kDa gastrin-binding protein; TP-alpha; HADH; anti-HADHA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HADHA polyclonal antibody detects endogenous levels of HADHA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 763

• Applicable Applications for anti-HADHA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human HADHA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of HADHA polyclonal antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of HADHA polyclonal antibody in paraffin-embedded Human Thyroid Cancer tissue.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells, using HADHA polyclonal antibody)

Related Product Information for anti-HADHA antibody

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.

NCBI and Uniprot Product Information

• NCBI GI #: 20127408
• NCBI GeneID: 3030
• NCBI Accession #: NP_000173
• NCBI GenBank Nucleotide #: NP_000173.2
• UniProt Accession #: P40939
• Molecular Weight: ~83kDa
• NCBI Official Full Name: trifunctional enzyme subunit alpha, mitochondrial
• NCBI Official Synonym Full Names: hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
• NCBI Official Symbol: HADHA
• NCBI Official Synonym Symbols: GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA
• NCBI Protein Information: trifunctional enzyme subunit alpha, mitochondrial
• UniProt Protein Name: Trifunctional enzyme subunit alpha, mitochondrial
• Protein Family: Trifunctional enzyme
• UniProt Gene Name: HADHA
• UniProt Synonym Gene Names: HADH
• UniProt Entry Name: ECHA_HUMAN

NCBI Description

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008]