Western Blot (WB)
(A predominant 75 kDa band for the HEK293T wild type lysate was observed (3 ug/ml anti-HADHA) vs the predicted size of 83 kDa. The molecular weight discrepancy could be due to post-translationally modification of the target protein, a splice-variant form of the target protein, a partially degraded form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A less intense band at 65 kDa was also A weak band only in the 20ug lane was observed for the knock out lysate, suggesting incomplete knockout of the target gene.)
Rabbit anti-Human HADHA Polyclonal Antibody | anti-HADHA antibody
HADHA Antibody (C-term) [Knockout Validated]
IHC-P: 1:10-50
FC/FACS: 1:10-50
Western Blot (WB)
(A predominant 75 kDa band for the HEK293T wild type lysate was observed (3 ug/ml anti-HADHA) vs the predicted size of 83 kDa. The molecular weight discrepancy could be due to post-translationally modification of the target protein, a splice-variant form of the target protein, a partially degraded form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A less intense band at 65 kDa was also A weak band only in the 20ug lane was observed for the knock out lysate, suggesting incomplete knockout of the target gene.)
Western Blot (WB)
(A predominant 75 kDa band for the HEK293T wild type lysate was observed (3 ug/ml anti-HADHA) vs the predicted size of 83 kDa. The molecular weight discrepancy could be due to post-translationally modification of the target protein, a splice-variant form of the target protein, a partially degraded form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A less intense band at 65 kDa was also A weak band only in the 20ug lane was observed for the knock out lysate, suggesting incomplete knockout of the target gene.)
Western Blot (WB)
(All lanes : Anti-HADHA Antibody (C-term) at 1:1000 dilutionLane 1: Hela whole cell lysateLane 2: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-HADHA Antibody (C-term) at 1:1000 dilutionLane 1: Hela whole cell lysateLane 2: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(HADHA Antibody (C-term) (Cat.# AP6882b) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HADHA antibody detected the HADHA protein (arrow).)
Western Blot (WB)
(HADHA Antibody (C-term) (Cat.# AP6882b) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HADHA antibody detected the HADHA protein (arrow).)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(HADHA Antibody (C-term) (Cat. #AP6882b) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(HADHA Antibody (C-term) (Cat. #AP6882b) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Background: HADHA is the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008]
Research Articles on HADHA
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Product Notes
The HADHA hadha (Catalog #AAA9232249) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HADHA Antibody (C-term) [Knockout Validated] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HADHA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS). WB: 1:1000 IHC-P: 1:10-50 FC/FACS: 1:10-50. Researchers should empirically determine the suitability of the HADHA hadha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HADHA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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