Western Blot (WB)
(Western blot analysis of Jurkat whole cell lysates, using HADHA Antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit HADHA Polyclonal Antibody | anti-HADHA antibody
HADHA Antibody
Gene Names
HADHA; GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HADHA; Polyclonal Antibody; HADHA Antibody; 3 ketoacyl Coenzyme A (CoA) thiolase alpha subunit; 3 oxoacyl CoA thiolase; 78 kDa gastrin binding protein; 78 kDa gastrin-binding protein; ECHA; ECHA_HUMAN; GBP; HADH; Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit; LCEH; LCHAD; Long chain 3-hydroxyacyl-CoA dehydrogenase; Mitochondrial long chain 2 enoyl Coenzyme A (CoA) hydratase alpha subunit; Mitochondrial long chain L 3 hydroxyacyl Coenzyme A dehydrogenase alpha subunit; Mitochondrial trifunctional enzyme alpha subunit; Mitochondrial trifunctional protein alpha subunit; MTPA; Thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit; TP ALPHA; TP-alpha; Trifunctional enzyme subunit alpha mitochondrial precursor; anti-HADHA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HADHA antibody detects endogenous levels of total HADHA
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
763
Applicable Applications for anti-HADHA antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human HADHA
Subcellular Location
Mitochondrion.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Jurkat whole cell lysates, using HADHA Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of Jurkat whole cell lysates, using HADHA Antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9607926 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9607926 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts from rat brain, using HADHA Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat brain, using HADHA Antibody.)
Related Product Information for anti-HADHA antibody
Description: This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
Function: Bifunctional subunit.
Subunit Structure: Octamer of 4 alpha (HADHA) and 4 beta (HADHB) subunits.
Similarity: In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
Function: Bifunctional subunit.
Subunit Structure: Octamer of 4 alpha (HADHA) and 4 beta (HADHB) subunits.
Similarity: In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 83 kDa
Predicted: 83 kDa
Predicted: 83 kDa
NCBI Official Full Name
trifunctional enzyme subunit alpha, mitochondrial
NCBI Official Synonym Full Names
hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
NCBI Official Symbol
HADHA
NCBI Official Synonym Symbols
GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA
NCBI Protein Information
trifunctional enzyme subunit alpha, mitochondrial
UniProt Protein Name
Trifunctional enzyme subunit alpha, mitochondrial
Protein Family
UniProt Gene Name
HADHA
UniProt Synonym Gene Names
HADH
NCBI Description
This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008]
Uniprot Description
Bifunctional subunit.
Research Articles on HADHA
Similar Products
Product Notes
The HADHA hadha (Catalog #AAA9607926) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HADHA Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HADHA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the HADHA hadha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HADHA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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