Rabbit GZMM Polyclonal Antibody | anti-GZMM antibody

GZMM Polyclonal Antibody

Cat. Num. AAA3007167

• Gene Names: GZMM; MET1; LMET1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GZMM; Polyclonal Antibody; GZMM Polyclonal Antibody; Granzyme M (EC: 3.4.21.-); Met-1 serine protease; Hu-Met-1; Met-ase; Natural killer cell granular protease; MET1; anti-GZMM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GZMM polyclonal antibody detects endogenous levels of GZMM protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 218

• Applicable Applications for anti-GZMM antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human GZMM.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GZMM polyclonal antibody at 1:500 dilutionLine1:Hela whole cell lysateLine2:PC12 whole cell lysateLine3:sp20 whole cell lysate)

Related Product Information for anti-GZMM antibody

The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis, thereby mediating apoptosis through two different pathways. Granzyme H localizes to cytoplasmic granules of cytolytic T lymphocytes and is important for target cell lysis in cell-mediated immune responses. Granzyme K is a serine protease localizing to the granules of NK cells and cytotoxic T lymphocytes. Granzyme M (also designated NK cell granular protease, HU-Met-1 or met-1 serine protease) contains one peptidase S1 domain. Granzyme M is a Trypsin-fold serine protease that localizes to granules of large granular lymphocytes (NK cells) and cleaves peptide substrates after leucine, norleucine or methionine. This enzyme may play a role in target cell death induction by cytotoxic lymphocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 385275110
• NCBI GeneID: 3004
• NCBI Accession #: NP_001245280.1
• NCBI GenBank Nucleotide #: NP_001245280.1
• UniProt Accession #: P51124
• Molecular Weight: ~ 28kDa
• NCBI Official Full Name: granzyme M isoform 2
• NCBI Official Synonym Full Names: granzyme M
• NCBI Official Symbol: GZMM
• NCBI Official Synonym Symbols: MET1; LMET1
• NCBI Protein Information: granzyme M
• UniProt Protein Name: Granzyme M
• Protein Family: Granzyme
• UniProt Gene Name: GZMM
• UniProt Synonym Gene Names: MET1; Hu-Met-1

NCBI Description

Human natural killer (NK) cells and activated lymphocytes express and store a distinct subset of neutral serine proteases together with proteoglycans and other immune effector molecules in large cytoplasmic granules. These serine proteases are collectively termed granzymes and include 4 distinct gene products: granzyme A, granzyme B, granzyme H, and the protein encoded by this gene, granzyme M. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells.