Western Blot (WB)
(Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 16E4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~87kDa.)
Mouse anti-Mouse ENaC beta Monoclonal Antibody | anti-ENaC beta antibody
ENaC beta Antibody, Clone 16E4: PerCP
Gene Names
Scnn1b; RNENACB
Reactivity
Mouse
Applications
Western Blot
Purity
Protein G Purified
Synonyms
ENaC beta; Monoclonal Antibody; ENaC beta Antibody; Clone 16E4: PerCP; Mouse Anti-Rat ENaC beta Monoclonal IgG2a; SCNN1B Antibody; Amiloride sensitive sodium channel subunit beta Antibody; Amiloride-sensitive sodium channel beta-subunit Antibody; Beta ENaC Antibody; Beta NaCH Antibody; ENaCB Antibody; Epithelial Na(+) channel subunit beta Antibody; Epithelial Na+ channel beta subunit Antibody; Epithelial Na+ channel subunit beta Antibody; Epithelial sodium channel beta 2 subunit Antibody; Epithelial sodium channel beta 3 subunit Antibody; Nonvoltage gated sodium channel 1 beta subunit Antibody; Nonvoltage gated sodium channel 1 subunit beta Antibody; Nonvoltage-gated sodium channel 1 beta subunit Antibody; SCNEB Antibody; SCNN 1B Antibody; Sodium channel nonvoltage gated 1 beta (Liddle syndrome) Antibody; Sodium channel nonvoltage gated 1 beta Antibody; anti-ENaC beta antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
16E4
Specificity
Detects ~87kDa.
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
638
Applicable Applications for anti-ENaC beta antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Target
ENaC beta
Immunogen
Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)
Immunogen Species
Rat
Cellular Localization
Apical Cell Membrane
Conjugate
PerCP
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 16E4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~87kDa.)
Western Blot (WB)
(Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 16E4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~87kDa.)
Related Product Information for anti-ENaC beta antibody
The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2).
ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
Product Categories/Family for anti-ENaC beta antibody
References
1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,995 Da
NCBI Official Full Name
amiloride-sensitive sodium channel subunit beta
NCBI Official Synonym Full Names
sodium channel epithelial 1 beta subunit
NCBI Official Symbol
Scnn1b
NCBI Official Synonym Symbols
RNENACB
NCBI Protein Information
amiloride-sensitive sodium channel subunit beta
UniProt Protein Name
Amiloride-sensitive sodium channel subunit beta
UniProt Gene Name
Scnn1b
UniProt Synonym Gene Names
Beta-ENaC
NCBI Description
acts as an epithelial sodium ion channel; regulates salt and fluid transport in the kidney [RGD, Feb 2006]
Uniprot Description
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Research Articles on ENaC beta
Similar Products
Product Notes
The ENaC beta scnn1b (Catalog #AAA808319) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ENaC beta Antibody, Clone 16E4: PerCP reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ENaC beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the ENaC beta scnn1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENaC beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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