Western Blot (WB)
(Western Blot analysis of GTSF1 on zebrafish lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:200 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.)
Rabbit anti-Zebrafish GTSF1 Polyclonal Antibody | anti-GTSF1 antibody
GTSF1 Polyclonal Antibody
Reactivity
Zebrafish
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Protein affinity purified.
Synonyms
GTSF1; Polyclonal Antibody; GTSF1 Polyclonal Antibody; Gtsf1; 56699; Cue110; Fam112b; Family with sequence similarity 112 member B; FLJ32942; Gametocyte-specific factor 1; Protein FAM112B antibody; anti-GTSF1 antibody
Host
Rabbit
Reactivity
Zebrafish
Clonality
Polyclonal
Isotype
IgG
Specificity
GTSF1 polyclonal antibody detects endogenous levels of GTSF1 protein.
Purity/Purification
Protein affinity purified.
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-GTSF1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein within zebrafish GTSF1 aa 20-170.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of GTSF1 on zebrafish lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:200 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.)
Western Blot (WB)
(Western Blot analysis of GTSF1 on zebrafish lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:200 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.)
Immunofluorescence (IF)
(Immunofluorescence staining of paraffin- embedded Zebrafish using anti-GTSF1 rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9. 0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1803-68 at 1/50 dilution for 10 hours at 4 degree C and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.)
Immunofluorescence (IF)
(Immunofluorescence staining of paraffin- embedded Zebrafish using anti-GTSF1 rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9. 0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1803-68 at 1/50 dilution for 10 hours at 4 degree C and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.)
Related Product Information for anti-GTSF1 antibody
Gametocyte-specific factor 1 (Gtsf1), also known as the computationally obtained undifferentiated and/or embryonic stem cell-specific 110 gene (Cue110). Gtsf1 encodes a 167-amino acid protein that is a member of the Uncharacterized Protein Family 0224. DmGTSF1 is essential for P-element-induced wimpy testis (PIWI)-interacting RNA (piRNA)-mediated transcriptional repression and histone H3K9me3-mediated repression of transposons and their neighboring genes in the ovary. DmGTSF1 interacts with Piwi via its C-terminal tail and the two CHHC zinc-finger motifs within the DmGTSF1 protein are required for its activity. The Gtsf1 gene is essential for spermatogenesis and has been revealed to function in transposon suppression in the mouse testes. GTSF1 transcript expression has been observed to be up-regulated in certain leukemias and in lymphomas.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
35kDa
Predicted Band Size: 21kDa
Predicted Band Size: 21kDa
Protein Family
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Product Notes
The GTSF1 (Catalog #AAA3012793) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GTSF1 Polyclonal Antibody reacts with Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's GTSF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:200 IF: 1:50-1:200 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GTSF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTSF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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