Rabbit anti-Mouse GTF2H1 Polyclonal Antibody | anti-GTF2H1 antibody

GTF2H1, NT (GTF2H1, BTF2, General transcription factor IIH subunit 1, Basic transcription factor 2 62kD subunit, General transcription factor IIH polypeptide 1, TFIIH basal transcription factor complex p62 subunit) (FITC)

Cat. Num. AAA6304880

• Gene Names: GTF2H1; P62; BTF2; TFB1; TFIIH
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GTF2H1; Polyclonal Antibody; NT (GTF2H1; BTF2; General transcription factor IIH subunit 1; Basic transcription factor 2 62kD subunit; General transcription factor IIH polypeptide 1; TFIIH basal transcription factor complex p62 subunit) (FITC); TFIIH basal transcription factor complex p62 subunit; anti-GTF2H1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GTF2H1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GTF2H1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-112 amino acids from the N-terminal region of human GTF2H1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GTF2H1 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GTF2H1 antibody detected the GTF2H1 protein (arrow).)

Related Product Information for anti-GTF2H1 antibody

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.

Product Categories/Family for anti-GTF2H1 antibody

Antibodies; Transcription Factors

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). Wu, W., et al. Lung Cancer 63(2):180-186(2009). Bethke, L., et al. J. Natl. Cancer Inst. 100(4):270-276(2008). Di Lello, P., et al. Biochemistry 44(21):7678-7686(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 214831601
• NCBI GeneID: 2965
• NCBI Accession #: NP_001135779.1; NP_005307.1
• NCBI GenBank Nucleotide #: NM_001142307.1
• UniProt Accession #: P32780; Q6I9Y7; Q9H5K5; Q9NQD9; D3DQY2; AAB23294
• Molecular Weight: 62,032 Da
• NCBI Official Full Name: general transcription factor IIH subunit 1
• NCBI Official Synonym Full Names: general transcription factor IIH, polypeptide 1, 62kDa
• NCBI Official Symbol: GTF2H1
• NCBI Official Synonym Symbols: P62; BTF2; TFB1; TFIIH
• NCBI Protein Information: general transcription factor IIH subunit 1; BTF2 p62; basic transcription factor 2 62 kDa subunit; general transcription factor IIH polypeptide 1; TFIIH basal transcription factor complex p62 subunit
• UniProt Protein Name: General transcription factor IIH subunit 1
• Protein Family: General transcription factor
• UniProt Gene Name: GTF2H1
• UniProt Synonym Gene Names: BTF2; BTF2 p62
• UniProt Entry Name: TF2H1_HUMAN

Uniprot Description

GTF2H1: Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 11p15.1-p14

Cellular Component: nucleoplasm; holo TFIIH complex

Molecular Function: RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; protein binding; protein kinase activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; protein amino acid phosphorylation; regulation of gene expression, epigenetic; mRNA capping; nucleotide-excision repair; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; nucleotide-excision repair, DNA damage removal; positive regulation of transcription from RNA polymerase II promoter; regulation of cyclin-dependent protein kinase activity; transcription initiation from RNA polymerase I promoter