Rabbit 14-3-3 beta Polyclonal Antibody | anti-14-3-3 antibody

14-3-3 beta (Q69) Polyclonal Antibody

Cat. Num. AAA3002237

• Gene Names: YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: 14-3-3 beta; Polyclonal Antibody; 14-3-3 beta (Q69) Polyclonal Antibody; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1; YWHAB; 14-3-3beta; 14-3-3 alpha; 14-3-3alpha; 1433B; 14-3-3 beta/alpha; 14-3-3beta/alpha; anti-14-3-3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: 14-3-3 beta (Q69) polyclonal antibody detects endogenous levels of 14-3-3 beta protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 246

• Applicable Applications for anti-14-3-3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 40-90 of Human 14-3-3 beta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of 14-3-3 beta (Q69) polyclonal antibody at 1:500 dilutionLane1:U-87MG whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug) Lane6:PC12 whole cell lysate (40ug) Lane7:CT-26 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of 14-3-3 beta (Q69) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-14-3-3 antibody

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, epsilon, zeta, eta, theta and sigma. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

NCBI and Uniprot Product Information

• NCBI GI #: 4507949
• NCBI GeneID: 7529
• NCBI Accession #: NP_003395.1
• NCBI GenBank Nucleotide #: NP_003395.1
• UniProt Accession #: P31946
• Molecular Weight: ~ 28kDa
• NCBI Official Full Name: 14-3-3 protein beta/alpha
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
• NCBI Official Symbol: YWHAB
• NCBI Official Synonym Symbols: HS1; GW128; YWHAA; KCIP-1; HEL-S-1
• NCBI Protein Information: 14-3-3 protein beta/alpha
• UniProt Protein Name: 14-3-3 protein beta/alpha
• UniProt Gene Name: YWHAB
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433B_HUMAN

NCBI Description

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; transcriptional repressor complex; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; histone deacetylase binding; protein complex binding; phosphoprotein binding; transcription corepressor activity; phosphoserine binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of protein amino acid dephosphorylation; positive regulation of catalytic activity; axon guidance; fibroblast growth factor receptor signaling pathway; activation of MAPKK activity; nerve growth factor receptor signaling pathway; apoptosis; protein heterooligomerization; MAPKKK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; cytoplasmic sequestering of protein; innate immune response; gene expression; vascular endothelial growth factor receptor signaling pathway; negative regulation of transcription, DNA-dependent; protein targeting