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Western Blot (WB) (Western blot analysis of GSTA2 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). GSTA2 (arrow) was detected using the purified Pab.)

Rabbit anti-Human GSTA2 Polyclonal Antibody | anti-GSTA2 antibody

GSTA2, NT (GSTA2, GST2, Glutathione S-transferase A2, GST HA subunit 2, GST class-alpha member 2, GST-gamma, GSTA2-2, GTH2) (Biotin)

Gene Names
GSTA2; GST2; GTA2; GTH2; GSTA2-2
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GSTA2; Polyclonal Antibody; NT (GSTA2; GST2; Glutathione S-transferase A2; GST HA subunit 2; GST class-alpha member 2; GST-gamma; GSTA2-2; GTH2) (Biotin); GTH2; anti-GSTA2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-GSTA2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
GSTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human GSTA2.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GSTA2 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). GSTA2 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of GSTA2 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). GSTA2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GSTA2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GSTA2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Product Categories/Family for anti-GSTA2 antibody
References
Tars, K., et al. J. Mol. Biol. 397(1):332-340(2010). Moyer, A.M., et al. Cancer Epidemiol. Biomarkers Prev. 19(3):811-821(2010). Gemignani, F., et al. Mutat. Res. 671 (1-2), 76-83 (2009). Rohrdanz, E., et al. Arch. Biochem. Biophys. 298(2):747-752(1992) Bogaards, J.J., et al. Biochem. J. 286 (PT 2), 383-388 (1992) Klone, A., et al. Biochem. J. 285 (PT 3), 925-928 (1992).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,664 Da
NCBI Official Full Name
glutathione S-transferase A2
NCBI Official Synonym Full Names
glutathione S-transferase alpha 2
NCBI Official Symbol
GSTA2
NCBI Official Synonym Symbols
GST2; GTA2; GTH2; GSTA2-2
NCBI Protein Information
glutathione S-transferase A2
UniProt Protein Name
Glutathione S-transferase A2
Protein Family
UniProt Gene Name
GSTA2
UniProt Synonym Gene Names
GST2

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq, Jul 2008]

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Research Articles on GSTA2

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Product Notes

The GSTA2 gsta2 (Catalog #AAA6304692) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GSTA2, NT (GSTA2, GST2, Glutathione S-transferase A2, GST HA subunit 2, GST class-alpha member 2, GST-gamma, GSTA2-2, GTH2) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GSTA2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GSTA2 gsta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSTA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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