Rabbit anti-Mouse GSK3B Polyclonal Antibody | anti-GSK3B antibody

Anti-GSK3B Antibody, Rabbit Polyclonal

Cat. Num. AAA8111879

• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: GSK3B; Polyclonal Antibody; Anti-GSK3B Antibody; Rabbit Polyclonal; GSK3B Antibody; Rabbit PAb; Antigen Affinity Purified; glycogen synthase kinase 3 beta; Anti-7330414F15Rik Antibody; Anti-8430431H08Rik Antibody; Anti-C86142 Antibody; Anti-GSK-3 Antibody; Anti-GSK-3beta Antibody; Anti-GSK3 Antibody; anti-GSK3B antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse GSK3B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GSK3B antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1000-1:5000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Mouse GSK3B Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse GSK3B (rM GSK3B). GSK3B specific IgG was purified by Mouse GSK3B affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-GSK3B rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A549 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:49 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of GSK3B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human GSK3B polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

Immunoprecipitation (IP)

(Mouse GSK3B was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-Mouse GSK3B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse GSK3B rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 47 kDaObserved band size: 47 kDa)

Related Product Information for anti-GSK3B antibody

GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It Contains 1 protein kinase domain, and is expressed in the testis, thymus, prostate, and ovary and weakly expressed in the lung, brain, and kidney. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in the GSK3B gene have been implicated in modifying the risk of Parkinson's disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer's disease. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB, and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which are critical for its role in bulge stem cell migration and skin wound repair. It may be required for early embryo development and neuron differentiation.

Product Categories/Family for anti-GSK3B antibody

Notch Pathway; Canonical Wnt Pathway

References

Bergmann C, et al. (2011) Inhibition of glycogen synthase kinase 3beta induces dermal fibrosis by activation of the canonical Wnt pathway. Ann Rheum Dis. 70(12):2191-8.Ban JO, et al. (2011) Troglitazone, a PPAR agonist, inhibits human prostate cancer cell growth through inactivation of NFkappaB via suppression of GSK-3beta expression. Cancer Biol Ther. 12(4):288-96.Tsukigi M, et al. (2012) Re-expression of miR-199a suppresses renal cancer cell proliferation and survival by targeting GSK-3beta. Cancer Lett. 315(2):189-97.Nandan D, et al. (2012) Myeloid cell IL-10 production in response to leishmania involves inactivation of glycogen synthase kinase-3beta downstream of phosphatidylinositol-3 kinase. J Immunol. 188(1):367-78.

NCBI and Uniprot Product Information

• UniProt Accession #: Q9WV60
• Protein Family: Glycogen synthase kinase