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Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse NUAK1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse NUAK Family SNF1 Like Kinase 1 Polyclonal Antibody | anti-NUAK1 antibody

Polyclonal Antibody to NUAK Family SNF1 Like Kinase 1 (NUAK1)

Gene Names
Nuak1; Omphk1; AU014801; AW494241; B230104P22Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
NUAK Family SNF1 Like Kinase 1; Polyclonal Antibody; Polyclonal Antibody to NUAK Family SNF1 Like Kinase 1 (NUAK1); anti-NUAK1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NUAK1. It has beenselected for its ability to recognize NUAK1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
661
Applicable Applications for anti-NUAK1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
NUAK1 (Lys404~Leu651)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse, Rat
Conjugate
No Conjugate
Immunogen
Recombinant NUAK1 (Lys404~Leu651) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083505
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse NUAK1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse NUAK1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,661 Da
NCBI Official Full Name
NUAK family SNF1-like kinase 1
NCBI Official Synonym Full Names
NUAK family, SNF1-like kinase, 1
NCBI Official Symbol
Nuak1
NCBI Official Synonym Symbols
Omphk1; AU014801; AW494241; B230104P22Rik
NCBI Protein Information
NUAK family SNF1-like kinase 1
UniProt Protein Name
NUAK family SNF1-like kinase 1
UniProt Gene Name
Nuak1
UniProt Synonym Gene Names
Kiaa0537; Omphk1

Uniprot Description

NuaK1: a protein kinase of the CAMKL family. Phosphorylated and activated by AKT1 during glucose starvation. May activate ATM kinase under conditions of nutrient starvation. Suppresses apoptosis induced by nutrient starvation and death receptors via inhibition of caspase 8 activation.

Protein type: CAMK group; CAMKL family; EC 2.7.11.1; Kinase, protein; NuaK subfamily; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10|10 C1

Cellular Component: cytoplasm; fibrillar center; microtubule cytoskeleton; nucleus

Molecular Function: ATP binding; kinase activity; nucleotide binding; p53 binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity

Biological Process: cell adhesion; cellular response to DNA damage stimulus; phosphorylation; protein amino acid phosphorylation; regulation of cell adhesion; regulation of cellular senescence

Research Articles on NUAK1

Similar Products

Product Notes

The NUAK1 nuak1 (Catalog #AAA2026096) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to NUAK Family SNF1 Like Kinase 1 (NUAK1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NUAK Family SNF1 Like Kinase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the NUAK1 nuak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUAK Family SNF1 Like Kinase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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