Rabbit NFE2L2 Polyclonal Antibody | anti-NFE2L2 antibody

NFE2L2 Antibody

Cat. Num. AAA9401873

• Gene Names: NFE2L2; NRF2; HEBP1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: NFE2L2; Polyclonal Antibody; NFE2L2 Antibody; NRF2; anti-NFE2L2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total NFE2L2 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 589

• Applicable Applications for anti-NFE2L2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human NFE2L2.
• Target Name: NFE2L2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NFE2L2 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using NFE2L2 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-NFE2L2 antibody

The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintains cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5).

Product Categories/Family for anti-NFE2L2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 224028257
• NCBI GeneID: 4780
• NCBI Accession #: NP_001138884.1
• NCBI GenBank Nucleotide #: NM_001145412.3
• UniProt Accession #: Q16236; Q53RW6; Q59HH2; Q96F71; B2RBU2; B4E338; E9PGJ7
• Molecular Weight: 65,356 Da
• NCBI Official Full Name: nuclear factor erythroid 2-related factor 2 isoform 2
• NCBI Official Synonym Full Names: nuclear factor, erythroid 2-like 2
• NCBI Official Symbol: NFE2L2
• NCBI Official Synonym Symbols: NRF2; HEBP1
• NCBI Protein Information: nuclear factor erythroid 2-related factor 2
• UniProt Protein Name: Nuclear factor erythroid 2-related factor 2
• Protein Family: Nuclear factor erythroid 2-related factor
• UniProt Gene Name: NFE2L2
• UniProt Synonym Gene Names: NRF2; NF-E2-related factor 2; NFE2-related factor 2
• UniProt Entry Name: NF2L2_HUMAN

NCBI Description

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: nucleoplasm; centrosome; cytoplasm; plasma membrane; nucleus; chromatin; cytosol

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; positive regulation of blood coagulation; unfolded protein response; regulation of embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter