Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Macrophage erythroblast attacher (MAEA) Standard Curve.)
Rat Macrophage Erythroblast Attacher (MAEA) ELISA Kit | MAEA elisa kit
Rat Macrophage Erythroblast Attacher (MAEA) ELISA Kit
Reactivity
Rat
Synonyms
Macrophage Erythroblast Attacher (MAEA); Rat Macrophage Erythroblast Attacher (MAEA) ELISA Kit; MAEA; EMLP; EMP; HLC-10; PIG5; erythroblast macrophage protein; lung cancer-related protein 10; proliferation-inducing gene 5; MAEA elisa kit
Reactivity
Rat
Specificity
Rat Macrophage erythroblast attacher (MAEA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAEA. No significant cross-reactivity or interference between Rat MAEA and analogues was observed.
Sequence Length
396
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Macrophage erythroblast attacher (MAEA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Macrophage erythroblast attacher (MAEA) Standard Curve.)
Related Product Information for MAEA elisa kit
Description: This Rat Macrophage erythroblast attacher (MAEA) ELISA Kit employs a two-site sandwich ELISA to quantitate MAEA in samples. An antibody specific for MAEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAEA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAEA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAEA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Macrophage Erythroblast Attacher mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.
Background: Macrophage Erythroblast Attacher mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
E3 ubiquitin-protein transferase MAEA
NCBI Official Synonym Full Names
macrophage erythroblast attacher
NCBI Official Symbol
Maea
NCBI Protein Information
E3 ubiquitin-protein transferase MAEA
UniProt Protein Name
Macrophage erythroblast attacher
Protein Family
UniProt Gene Name
Maea
UniProt Entry Name
MAEA_RAT
Similar Products
Product Notes
The Rat MAEA maea (Catalog #AAA9712344) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712344 ELISA Kit recognizes Rat MAEA. It is sometimes possible for the material contained within the vial of "Macrophage Erythroblast Attacher (MAEA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
