Rabbit GRK 3/ADRBK2 Polyclonal Antibody | anti-GRK3 antibody

GRK 3/ADRBK2 Polyclonal Antibody

Cat. Num. AAA3008001

• Gene Names: GRK3; BARK2; ADRBK2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GRK 3/ADRBK2; Polyclonal Antibody; GRK 3/ADRBK2 Polyclonal Antibody; G-protein coupled receptor kinase 3; Beta-adrenergic receptor kinase 2; Beta-ARK-2; GRK3; BARK2; anti-GRK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADRBK2 polyclonal antibody detects endogenous levels of ADRBK2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 688

• Applicable Applications for anti-GRK3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human GRK 3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of GRK 3 /ADRBK2 polyclonal)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of GRK 3 /ADRBK2 polyclonal in paraffin-embedded Human Kidney Cancer tissue.)

Related Product Information for anti-GRK3 antibody

Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonist-mediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messenger-regulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1), two forms of beta-adrenergic receptor kinase: GRK 2 (betaARK, betaARK1) and GRK 3 (betaARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

NCBI and Uniprot Product Information

• NCBI GI #: 148539879
• NCBI GeneID: 157
• NCBI Accession #: NP_005151.2
• NCBI GenBank Nucleotide #: NP_005151.2
• UniProt Accession #: P35626
• Molecular Weight: ~ 100kDa
• NCBI Official Full Name: beta-adrenergic receptor kinase 2
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 3
• NCBI Official Symbol: GRK3
• NCBI Official Synonym Symbols: BARK2; ADRBK2
• NCBI Protein Information: beta-adrenergic receptor kinase 2
• UniProt Protein Name: Beta-adrenergic receptor kinase 2
• UniProt Gene Name: ADRBK2
• UniProt Synonym Gene Names: BARK2; GRK3; Beta-ARK-2
• UniProt Entry Name: ARBK2_HUMAN

NCBI Description

The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]

Uniprot Description

GRK3: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.15; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 22q12.1

Molecular Function: G-protein coupled receptor kinase activity; beta-adrenergic receptor kinase activity; ATP binding; protein kinase activity

Biological Process: receptor internalization; signal transduction; protein amino acid phosphorylation