Rabbit GRK 2/ADRBK1 Polyclonal Antibody | anti-GRK2 antibody

GRK 2/ADRBK1 Polyclonal Antibody

Cat. Num. AAA3008248

• Gene Names: GRK2; BARK1; ADRBK1; BETA-ARK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GRK 2/ADRBK1; Polyclonal Antibody; GRK 2/ADRBK1 Polyclonal Antibody; Beta-adrenergic receptor kinase 1; Beta-ARK-1; beta-ARK-1; ARK-beta1; G-protein coupled receptor kinase 2; ADRBK1; BARK; BARK1; GRK2; ARK beta1; ARKbeta1; beta-ARK1; anti-GRK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADRBK1 polyclonal antibody detects endogenous levels of ADRBK1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 689

• Applicable Applications for anti-GRK2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human GRK 2 / ADRBK1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ADRBK1 pAb)

Related Product Information for anti-GRK2 antibody

Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second messenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1; two forms of beta-adrenergic receptor kinase, GRK 2 (betaARK, betaARK1) and GRK 3 (betaARK2); IT-11 (GRK 4); GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

NCBI and Uniprot Product Information

• NCBI GI #: 148539876
• NCBI GeneID: 156
• NCBI Accession #: NP_001610.2
• NCBI GenBank Nucleotide #: NP_001610.2
• UniProt Accession #: P25098
• Molecular Weight: ~ 80kDa
• NCBI Official Full Name: beta-adrenergic receptor kinase 1
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 2
• NCBI Official Symbol: GRK2
• NCBI Official Synonym Symbols: BARK1; ADRBK1; BETA-ARK1
• NCBI Protein Information: beta-adrenergic receptor kinase 1
• UniProt Protein Name: Beta-adrenergic receptor kinase 1
• Protein Family: Beta-adrenergic receptor kinase
• UniProt Gene Name: ADRBK1
• UniProt Synonym Gene Names: BARK; BARK1; GRK2; Beta-ARK-1
• UniProt Entry Name: ARBK1_HUMAN

NCBI Description

This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]

Uniprot Description

GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.15; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: G-protein coupled receptor kinase activity; protein binding; Edg-2 lysophosphatidic acid receptor binding; beta-adrenergic receptor kinase activity; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; acetylcholine receptor signaling, muscarinic pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; positive regulation of catecholamine secretion; innate immune response; cardiac muscle contraction