Mouse anti-Human S100A1 Monoclonal Antibody | anti-S100A1 antibody

S100A1 (Astrocyte Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438258

• Gene Names: S100A1; S100; S100A; S100-alpha
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry
• Synonyms: S100A1; Monoclonal Antibody; S100A1 (Astrocyte Marker) Mouse Monoclonal Antibody; Bpb; NEF; S100-A1; S100 Alpha Chain; S100 Beta Chain; S100 Calcium Binding Protein A1; S100 Calcium Binding Protein B; S100 Calcium Binding Protein Beta Neural; anti-S100A1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: 4C4.9
• Specificity: S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B).This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 94

• Applicable Applications for anti-S100A1 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Immunofluorescence (1-2ug/ml); Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Cellular Localization: Cytoplasmic
• Immunogen: Purified human S-100 protein conjugated to methylated BSA.
• Hu-Chromosome Location: 1q21.3
• Positive Control: Brain, Melanoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Mouse Monoclonal Antibody (S1/61))

Immunohistochemistry (IHC)

(Formalin--paraffin human Melanoma stained with S100A1 Monoclonal Antibody (4C4.9) (AEC Chromogen))

Western Blot (WB)

(Western Blot of human Cerebellum Lysate using S100A1 Monoclonal Antibody (4C4.9).)

Immunofluorescence (IF)

(Confocal Immunofluorescent analysis of A2058 cellsusing AF488-labeled S100A1 Monoclonal Antibody (4C4.9) (Green).)

Immunofluorescence (IF)

(Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green).)

References

McLaren KM et. al. Human Pathology, 1996, 27(7):633-6. Joshi MG et. al. Modern Pathology, 1996, 9(1):57-62.

NCBI and Uniprot Product Information

• NCBI GI #: 5454032
• NCBI GeneID: 6271
• NCBI Accession #: NP_006262.1
• NCBI GenBank Nucleotide #: NM_006271.1
• UniProt Accession #: P23297; Q5T7Y3; B2R5D9
• Molecular Weight: 10-12kDa
• NCBI Official Full Name: protein S100-A1
• NCBI Official Synonym Full Names: S100 calcium binding protein A1
• NCBI Official Symbol: S100A1
• NCBI Official Synonym Symbols: S100; S100A; S100-alpha
• NCBI Protein Information: protein S100-A1
• UniProt Protein Name: Protein S100-A1
• Protein Family: Protein
• UniProt Gene Name: S100A1
• UniProt Synonym Gene Names: S100A
• UniProt Entry Name: S10A1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A1: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. Belongs to the S-100 family.

Protein type: Endoplasmic reticulum; Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleus; sarcoplasmic reticulum

Molecular Function: ATPase binding; identical protein binding; protein binding; protein homodimerization activity

Biological Process: substantia nigra development