Western Blot (WB)
(Anti-GRB2 antibody, MBS175428, Western blottingWB: HELA Cell Lysate )
Rabbit GRB2 Polyclonal Antibody | anti-GRB2 antibody
Anti-GRB2 antibody
Gene Names
GRB2; ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
GRB2; Polyclonal Antibody; Anti-GRB2 antibody; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 2; Abundant SRC homology antibody; Adapter protein GRB2 antibody; ASH antibody; Ash protein antibody; EGFRBP GRB2 antibody; Epidermal growth factor receptor binding protein antibody; Epidermal growth factor receptor binding protein GRB2 antibody; GRB 2 antibody; GRB2 adapter protein antibody; Grb2 antibody; GRB2_HUMAN antibody; Grb3 3 antibody; Growth Factor Receptor Bound Protein 2 antibody; Growth factor receptor bound protein 3 antibody; Growth factor receptor-bound protein 2 antibody; HT027 antibody; MST084 antibody; MSTP084 antibody; NCKAP2 antibody; OTTHUMP00000166096 antibody; OTTHUMP00000166097 antibody; OTTHUMP00000166098 antibody; Protein ASH antibody; SEM5 antibody; SH2/SH3 adapter GRB2 antibody; anti-GRB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
217
Applicable Applications for anti-GRB2 antibody
Western Blot (WB)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GRB2 (184-201aa HVMDNSDPNWWKGACHGQ), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-GRB2 antibody, MBS175428, Western blottingWB: HELA Cell Lysate )
Western Blot (WB)
(Anti-GRB2 antibody, MBS175428, Western blottingWB: HELA Cell Lysate )
Related Product Information for anti-GRB2 antibody
Description: Rabbit IgG polyclonal antibody for Growth factor receptor-bound protein 2(GRB2) detection. Tested with WB in Human, Mouse, Rat.
Background: GRB2, Growth factor receptor-bound protein 2, is an adaptor protein involved in signal transduction/cell communication. The GRB2 gene encodes a protein that has homology to noncatalytic regions of the SRC oncogene product. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. In humans, the GRB2 protein is encoded by the GRB2 gene. Grb2 is widely expressed and is essential for multiple cellular functions. The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background: GRB2, Growth factor receptor-bound protein 2, is an adaptor protein involved in signal transduction/cell communication. The GRB2 gene encodes a protein that has homology to noncatalytic regions of the SRC oncogene product. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. In humans, the GRB2 protein is encoded by the GRB2 gene. Grb2 is widely expressed and is essential for multiple cellular functions. The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,557 Da
NCBI Official Full Name
Growth factor receptor-bound protein 2
NCBI Official Synonym Full Names
growth factor receptor-bound protein 2
NCBI Official Symbol
GRB2
NCBI Official Synonym Symbols
ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
NCBI Protein Information
growth factor receptor-bound protein 2; HT027; protein Ash; SH2/SH3 adapter GRB2; abundant SRC homology; growth factor receptor-bound protein 3; epidermal growth factor receptor-binding protein GRB2
UniProt Protein Name
Growth factor receptor-bound protein 2
Protein Family
UniProt Gene Name
GRB2
UniProt Synonym Gene Names
ASH
UniProt Entry Name
GRB2_HUMAN
NCBI Description
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Research Articles on GRB2
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Product Notes
The GRB2 grb2 (Catalog #AAA175428) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GRB2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GRB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the GRB2 grb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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