Western Blot (WB)
(WB Suggested Anti-POU1F1 Antibody Titration: 1.0ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate)
Rabbit POU1F1 Polyclonal Antibody | anti-POU1F1 antibody
POU1F1 antibody - N-terminal region
Western Blot (WB)
(WB Suggested Anti-POU1F1 Antibody Titration: 1.0ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate)
Western Blot (WB)
(WB Suggested Anti-POU1F1 Antibody Titration: 1.0ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate)
Target Description: PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
GHF-1: Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'- TAAAT-3'. Belongs to the POU transcription factor family. Class- 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 3p11.2
Cellular Component: nucleus; transcription factor complex
Molecular Function: chromatin binding; DNA binding transcription factor activity; transcription corepressor activity
Biological Process: B cell differentiation; cell fate specification; determination of adult lifespan; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; nuclear transport; positive regulation of cell proliferation; positive regulation of inositol trisphosphate biosynthetic process; positive regulation of multicellular organism growth; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of insulin-like growth factor receptor signaling pathway; regulation of transcription, DNA-templated; somatotropin secreting cell development; transcription from RNA polymerase II promoter
Disease: Pituitary Hormone Deficiency, Combined, 1
Research Articles on POU1F1
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Product Notes
The POU1F1 pou1f1 (Catalog #AAA3200421) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POU1F1 antibody - N-terminal region reacts with Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's POU1F1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the POU1F1 pou1f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: TPCLYKFPDH TLSHGFPPIH QPLLAEDPTA ADFKQELRRK SKLVEEPIDM. It is sometimes possible for the material contained within the vial of "POU1F1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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