Rabbit anti-Human Granzyme B Polyclonal Antibody | anti-GZMB antibody

Granzyme B, CT (GRB, GZMB, C11, Cathespin G-like 1, CCPI, CGL1, CGL-1, CSPB, CSP-B, CTLA1, CTLA-1, CTSGL1, Cytotoxic T Lymphocyte-associated Serine Esterase 1, Cytotoxic T Lymphocyte Proteinase 2, Fragmentin 2, Fragmentin-2, Granzyme 2, Human Lymphocyte P

Cat. Num. AAA6478897

• Gene Names: GZMB; C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Granzyme B; Polyclonal Antibody; CT (GRB; GZMB; C11; Cathespin G-like 1; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Cytotoxic T Lymphocyte-associated Serine Esterase 1; Cytotoxic T Lymphocyte Proteinase 2; Fragmentin 2; Fragmentin-2; Granzyme 2; Human Lymphocyte P; EC=3.4.21.79; Granzyme-2; Cytotoxic Serine Protease B; anti-GZMB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GZMB.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-GZMB antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GZMB (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GZMB Antibody (C-term) in HL-60 cell line lysates (35ug/lane). GZMB (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GZMB Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GZMB Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of GZMB (arrow) using rabbit polyclonal GZMB Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.)

Product Categories/Family for anti-GZMB antibody

Antibodies; Apoptosis

References

1. Hagn, M., J. Immunol. 183(3), 1838-1845 (2009). 2. Gaafar, A., Exp. Hematol. 37(7), 838-848 (2009). 3. Girnita, D.M., Transplantation 87(12), 1801-1806 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 221625528
• NCBI GeneID: 3002
• NCBI Accession #: NP_004122
• NCBI GenBank Nucleotide #: NM_004131.5
• UniProt Accession #: P10144; Q8N1D2; Q9UCC1
• Molecular Weight: 27.8kDa (248aa)
• NCBI Official Full Name: granzyme B isoform 1 preproprotein
• NCBI Official Synonym Full Names: granzyme B
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B
• UniProt Protein Name: Granzyme B
• Protein Family: Granzyme
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
• UniProt Entry Name: GRAB_HUMAN

NCBI Description

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]

Uniprot Description

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.79; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; immunological synapse; nucleus; cytosol

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; apoptosis; natural killer cell mediated cytotoxicity; cytolysis; protein processing; immune response; proteolysis